Trp548Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicides

Chen，Lei1,2,3; Gu，Gang2; Wang，Chengxu2; Chen，Zhufeng2; Yan，Wei1; Jin，Man2; Xie，Gang2; Zhou，Junli2; Deng，Xing Wang2,3; Tang，Xiaoyan1,2

2021

10.1016/j.cj.2020.11.003

Crop Journal   影响因子和分区

2095-5421

2214-5141

Herbicide resistance in crop plants is valuable for integrated weed management in agriculture. Herbicide resistant rice, in particular, is important to management of weedy rice, a close relative of cultivated rice and a noxious weed prevalent in rice fields that remains challenging to farmers worldwide. Herbicide resistant plants can be obtained through transgenic approach or by mutagenesis of regular plant and screening of mutants with elevated resistance to herbicide. In this study, we conducted ethyl methyl sulfonate mutagenesis (EMS) to elite indica cultivar Huanghuazhan (HHZ) and screened for mutants resistant to imazapic, a herbicide that can inhibit the acetolactate synthase (ALS) in plants. We obtained three mutants of OsALS gene that have not been reported previously in rice. One of the mutants, with Trp changed to Met (WM), was analyzed in more details in this study. This mutation had no negative effect on the plant physiology and morphology as well as rice yield. Compared with the imidazolinone-resistant mutant SN (Ser changed to Asn) that has been deployed for Clearfield rice development, WM mutant showed high levels of resistance to a broad spectrum of five families of ALS-inhibiting herbicides, in addition to a higher level of resistance to herbicides of the imidazolinone family. The herbicide-resistance was stably inherited by crossing into other rice lines. Thus, the WM mutation provides a valuable resource for breeding of herbicide resistant rice and weed management.

Acetolactate synthase ALS-inhibiting herbicide Herbicide tolerance Mutant Rice

SCI

英语

通讯

Major Program of Guangdong Basic and Applied Research[2019B030302006] ; National Natural Science Foundation of China["U1901203",31901532] ; Natural Science Foundation of Guangdong Province["2018B030308008","2018A0303130270"] ; Shenzhen Commission on Innovation and Technology Programs[JCYJ20180507181837997] ; China Postdoctoral Science Foundation["2018M633069","2019M652920"]

Agriculture ; Plant Sciences

Agronomy ; Plant Sciences

WOS:000685183000005

KEAI PUBLISHING LTD

2-s2.0-85099549262

Scopus

1.Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,School of Life Sciences,South China Normal University,Guangzhou,510631,China
2.Shenzhen Institute of Molecular Crop Design,Shenzhen,518107,China
3.Institute of Plant and Food Sciences,Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China

Chen，Lei,Gu，Gang,Wang，Chengxu,et al. Trp548Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicides[J]. Crop Journal,2021,9(4).

Chen，Lei.,Gu，Gang.,Wang，Chengxu.,Chen，Zhufeng.,Yan，Wei.,...&Tang，Xiaoyan.(2021).Trp548Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicides.Crop Journal,9(4).

Chen，Lei,et al."Trp548Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicides".Crop Journal 9.4(2021).