Ultrasound molecular imaging-guided tumor gene therapy through dual-targeted cationic microbubbles

Liu，Yingying1; Zhou，Yuli1; Xu，Jinfeng1; Luo，Hui1; Zhu，Yao1; Zeng，Xinxin1; Dong，Fajin1; Wei，Zhanghong1; Yan，Fei2; Zheng，Hairong2

2021-04-07

10.1039/d0bm01857k

Biomaterials Science   影响因子和分区

2047-4830

2047-4849

The success of gene therapy depends largely on the development of gene vectors and effective gene delivery systems. It has been demonstrated that cationic microbubbles can be loaded with negatively charged plasmid DNA and thus improve gene transfection efficiency. In this study, we developed dual-targeting cationic microbubbles conjugated with iRGD peptides(Cyclo(Cys-Arg-Gly-Asp-Lys-Gly-Pro-Asp-Cys)) and CCR2 (chemokine (C-C motif) receptor 2) antibodies (MBiRGD/CCR2) for ultrasound molecular imaging and targeted tumor gene therapy. The ultrasound molecular imaging experiments showed that there were significantly enhanced ultrasound molecular imaging signals in the tumor that received MBiRGD/CCR2, compared with those that received MBiRGD, MBCCR2, or MBcontrol. As a therapy plasmid, pGPU6/GFP/Neo-shAKT2, carrying an expression cassette for the human AKT2 RNA interference sequence, was used. Our results demonstrated that MBiRGD/CCR2 had a significantly higher gene transfection efficiency than MBiRGD, MBCCR2, or MBcontrol under ultrasound irradiation, resulting in much lower AKT2 protein expression and stronger tumor growth inhibition effects in vivo and in vitro. In conclusion, our study demonstrated a novel gene delivery system via MBiRGD/CCR2 for ultrasound molecular-imaging-guided gene therapy of breast cancer. This journal is

SCI ; EI

英语

第一 ; 通讯

WOS:000637697500031

2-s2.0-85103875298

Scopus

1.Shenzhen Medical Ultrasound Engineering Center,Department of Ultrasonography,Shenzhen People's Hospital,Second Clinical Medical College,Jinan University,First Clinical Medical College,Southern University of Science and Technology,Shenzhen,518020,China
2.Paul C. Lauterbur Research Center for Biomedical Imaging,Institute of Biomedical and Health Engineering,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China

Liu，Yingying,Zhou，Yuli,Xu，Jinfeng,et al. Ultrasound molecular imaging-guided tumor gene therapy through dual-targeted cationic microbubbles[J]. Biomaterials Science,2021,9(7):2454-2466.

Liu，Yingying.,Zhou，Yuli.,Xu，Jinfeng.,Luo，Hui.,Zhu，Yao.,...&Zheng，Hairong.(2021).Ultrasound molecular imaging-guided tumor gene therapy through dual-targeted cationic microbubbles.Biomaterials Science,9(7),2454-2466.

Liu，Yingying,et al."Ultrasound molecular imaging-guided tumor gene therapy through dual-targeted cationic microbubbles".Biomaterials Science 9.7(2021):2454-2466.