3D printed gelatin/hydroxyapatite scaffolds for stem cell chondrogenic differentiation and articular cartilage repair

Huang，Jianghong1,2; Huang，Zhiwang1; Liang，Yujie2,3; Yuan，Weihao4; Bian，Liming4; Duan，Li1,5; Rong，Zhibin6; Xiong，Jianyi1; Wang，Daping1,5,7; Xia，Jiang2

2021-04-07

10.1039/d0bm02103b

Biomaterials Science   影响因子和分区

2047-4830

2047-4849

Acute injury of the articular cartilage can lead to chronic disabling conditions because of the limited self-repair capability of the cartilage. Implantation of stem cells at the injury site is a viable treatment, but requires a scaffold with a precisely controlled geometry and porosity in the 3D space, high biocompatibility, and the capability of promoting chondrogenic differentiation of the implanted stem cells. Here we report the development of gelatin/hydroxyapatite (HAP) hybrid materials by microextrusion 3D bioprinting and enzymatic cross-linking as the scaffold for human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs). The scaffold supports the adhesion, growth, and proliferation of hUCB-MSCs and induces their chondrogenic differentiation in vitro. Doping HAP in the gelatin scaffold increases the fluidity of the hydrogel, improves the gelation kinetics and the rheological properties, and allows better control over 3D printing. Implanting the hUCB-MSC-laden scaffold at the injury site of the articular cartilage effectively repairs the cartilage defects in a pig model. Altogether, this work demonstrates the 3D printing of gelatin-based scaffold materials for hUCB-MSCs to repair cartilage defects as a potential treatment of articular cartilage injury. This journal is

SCI ; EI

英语

通讯

WOS:000637697500018

20211510207293

Biocompatibility ; Cartilage ; Cell culture ; Defects ; Flowcharting ; Gelation ; Hybrid materials ; Mammals ; Repair ; Scaffolds (biology) ; Stem cells

Bioengineering and Biology:461 ; Computer Programming:723.1 ; Printing Equipment:745.1.1 ; Chemical Operations:802.3 ; Maintenance:913.5 ; Materials Science:951

2-s2.0-85103568541

Scopus

1.Department of Orthopedics,Shenzhen Intelligent Orthopaedics and Biomedical Innovation Platform,Guangdong Artificial Intelligence Biomedical Innovation Platform,Shenzhen Second People's Hospital,First Affiliated Hospital,Shenzhen University Health Science Center,Shenzhen,518035,China
2.Department of Chemistry,Center for Cell and Developmental Biology,School of Life Sciences,Chinese University of Hong Kong,Shatin,Hong Kong
3.Shenzhen Kangning Hospital,Shenzhen Mental Health Center,Shenzhen Guangdong,518020,China
4.Department of Biomedical Engineering,Chinese University of Hong Kong,Shatin,Hong Kong
5.Guangzhou Medical University,Guangzhou Guangdong,511436,China
6.Shijiazhuang Maternity and Child Health Hospital,Shijiazhuang Hebei,050093,China
7.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China

Huang，Jianghong,Huang，Zhiwang,Liang，Yujie,et al. 3D printed gelatin/hydroxyapatite scaffolds for stem cell chondrogenic differentiation and articular cartilage repair[J]. Biomaterials Science,2021,9(7):2620-2630.

Huang，Jianghong.,Huang，Zhiwang.,Liang，Yujie.,Yuan，Weihao.,Bian，Liming.,...&Xia，Jiang.(2021).3D printed gelatin/hydroxyapatite scaffolds for stem cell chondrogenic differentiation and articular cartilage repair.Biomaterials Science,9(7),2620-2630.

Huang，Jianghong,et al."3D printed gelatin/hydroxyapatite scaffolds for stem cell chondrogenic differentiation and articular cartilage repair".Biomaterials Science 9.7(2021):2620-2630.