MiRNA-20b/SUFU/Wnt axis accelerates gastric cancer cell proliferation, migration and EMT

Peng，Yin1,2; Qin，Ying4; Zhang，Xiaojing1,2,3; Deng，Shiqi1; Yuan，Yuan1; Feng，Xianling1; Chen，Wangchun1; Hu，Fan1; Gao，Yuli1; He，Jieqiong1; Cheng，Yulan5; Wei，Yanjie6; Fan，Xinmin1; Ashktorab，Hassan7; Smoot，Duane8; Li，Song9; Meltzer，Stephen J.5; Zhuang，Shutong4; Tang，Na10; Jin，Zhe1,2

2021-04-01

10.1016/j.heliyon.2021.e06695

Heliyon   影响因子和分区

2405-8440

Previous research has found that miRNA-20b is highly expressed in gastric cancer (GC), however, its function and underlying mechanism are not clear. Wnt signaling pathway, implicated in tumorigeneisis, is activated in more than 30% of GC. We would like to characterize the biological behavior of miRNA-20b in terms of modulating Wnt/β-catenin signaling and EMT. We showed that miRNA-20b inhibitors suppressed Topflash/Fopflash dependent luciferase activity and the β-catenin nuclear translocation, resulting in inhibition of Wnt pathway activity and EMT. SUFU, negatively regulating Wnt and Hedgehog signaling pathway, was proved to be targeted by miRNA-20b. Moreover, additional knockdown of SUFU alleviated the inhibitory effect on Wnt pathway activity, EMT, cell proliferation/migration and colony formation caused by miRNA-20b inhibition. In summary, miRNA-20b is an oncogenic miRNA and promoted cell proliferation, migration and EMT in GC partially by activating Wnt pathway via targeting SUFU.

EMT miRNA-20b SUFU Wnt/β-catenin

SCI

英语

其他

WOS:000647558500034

2-s2.0-85104057525

Scopus

1.Guangdong Key Laboratory for Genome Stability & Disease Prevention,Department of Pathology,Shenzhen University School of Medicine,Shenzhen,518060,China
2.Guangdong Provincial Key Laboratory of Regional Immunity and Diseases,Department of Pathology,Health Science Center,Shenzhen University,Shenzhen,518055,China
3.Department of Pathology,Guangdong Province Key Laboratory of Molecular Oncologic Pathology,China
4.Department of Gastrointestinal Surgery,Shenzhen Second People's Hospital,Shenzhen,518000,China
5.Department of Medicine/GI Division,Johns Hopkins University School of Medicine and Sidney Kimmel Comprehensive Cancer Center,Baltimore,21287,United States
6.Center for High Performance Computing,Shenzhen Institutes of Advanced Technology,Shenzhen,518000,China
7.Department of Medicine and Cancer Center,Howard University,College of Medicine,Washington,20060,United States
8.Department of Medicine,Meharry Medical Center,Nashville,37208,United States
9.Shenzhen Science & Technology Development Exchange Center,Shenzhen,Shenzhen Science and Technology Building,518055,China
10.Department of Pathology,Shenzhen People's Hospital (The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,518020,China

Peng，Yin,Qin，Ying,Zhang，Xiaojing,et al. MiRNA-20b/SUFU/Wnt axis accelerates gastric cancer cell proliferation, migration and EMT[J]. Heliyon,2021,7(4).

Peng，Yin.,Qin，Ying.,Zhang，Xiaojing.,Deng，Shiqi.,Yuan，Yuan.,...&Jin，Zhe.(2021).MiRNA-20b/SUFU/Wnt axis accelerates gastric cancer cell proliferation, migration and EMT.Heliyon,7(4).

Peng，Yin,et al."MiRNA-20b/SUFU/Wnt axis accelerates gastric cancer cell proliferation, migration and EMT".Heliyon 7.4(2021).