Molecular Characterization of the Von Willebrand Factor Type D Domain of Vitellogenin from Takifugu flavidus

Qiao，Kun1; Jiang，Caiyun2; Xu，Min1; Chen，Bei1; Qiu，Wenhui3; Su，Yongchang1; Hao，Hua4; Lin，Zhiyong4; Cai，Shuilin1; Su，Jie1; Liu，Zhiyu1; Huang，Wenshu2

2021-03-25

10.3390/md19040181

Marine drugs   影响因子和分区

1660-3397

The von Willebrand factor type D (VWD) domain in vitellogenin has recently been found to bind tetrodotoxin. The way in which this protein domain associates with tetrodotoxin and participates in transporting tetrodotoxin in vivo remains unclear. A cDNA fragment of the vitellogenin gene containing the VWD domain from pufferfish (Takifugu flavidus) (TfVWD) was cloned. Using in silico structural and docking analyses of the predicted protein, we determined that key amino acids (namely, Val115, ASP116, Val117, and Lys122) in TfVWD mediate its binding to tetrodotoxin, which was supported by in vitro surface plasmon resonance analysis. Moreover, incubating recombinant rTfVWD together with tetrodotoxin attenuated its toxicity in vivo, further supporting protein-toxin binding and indicating associated toxicity-neutralizing effects. Finally, the expression profiling of TfVWD across different tissues and developmental stages indicated that its distribution patterns mirrored those of tetrodotoxin, suggesting that TfVWD may be involved in tetrodotoxin transport in pufferfish. For the first time, this study reveals the amino acids that mediate the binding of TfVWD to tetrodotoxin and provides a basis for further exploration of the molecular mechanisms underlying the enrichment and transfer of tetrodotoxin in pufferfish.

Takifugu flavidus tetrodotoxin toxin-binding protein transport vitellogenin VWD domain

SCI

英语

其他

basic research projects of provincial public welfare research institutes[2020R1013008] ; Seed Industry Innovation and Industrialization Project of Fujian Province[2017FJSCZY03] ; Special Funds of Fujian Ocean and Fisheries Structure Adjustment[2017HYJG07]

Pharmacology & Pharmacy

Chemistry, Medicinal ; Pharmacology & Pharmacy

WOS:000643210200001

MDPI

2-s2.0-85103862211

Scopus

1.Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province,Fisheries Research Institute of Fujian,Xiamen,361013,China
2.Fisheries College,Jimei University,Xiamen,361021,China
3.State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control,School of Environmental Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China
4.State Key Laboratory of Marine Environmental Science,College of Oceanography and Environmental Science,Xiamen University,Xiamen,361005,China

Qiao，Kun,Jiang，Caiyun,Xu，Min,et al. Molecular Characterization of the Von Willebrand Factor Type D Domain of Vitellogenin from Takifugu flavidus[J]. Marine drugs,2021,19(4).

Qiao，Kun.,Jiang，Caiyun.,Xu，Min.,Chen，Bei.,Qiu，Wenhui.,...&Huang，Wenshu.(2021).Molecular Characterization of the Von Willebrand Factor Type D Domain of Vitellogenin from Takifugu flavidus.Marine drugs,19(4).

Qiao，Kun,et al."Molecular Characterization of the Von Willebrand Factor Type D Domain of Vitellogenin from Takifugu flavidus".Marine drugs 19.4(2021).