南方科技大学知识苑(SUSTech KC): Advances in base editing with an emphasis on an AAV-based strategy

题名	Advances in base editing with an emphasis on an AAV-based strategy
作者	Kuang，Jiajie 1,2; Lyu，Qinghua 3,4; Wang，Jiao 3; Cui，Yubo 2; Zhao，Jun 1,2
通讯作者	Zhao，Jun
发表日期	2021
DOI	10.1016/j.ymeth.2021.03.015
发表期刊	METHODS 影响因子和分区
ISSN	1046-2023
EISSN	1095-9130
摘要	The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based base editors have been developed for precisely installing point mutations in genomes with high efficiency. Two editing systems of cytosine base editors (CBEs) and adenine base editors (ABEs) have been developed for conversion of C.G-to-T.A and A.T-to-G.C, respectively, showing the prominence in genomic DNA correction and mutation. Here, we summarize recent optimized approaches in improving base editors, including the evolution of Cas proteins, the choice of deamination enzymes, modification on linker length, base-editor expression, and addition of functional domains. Specifically, in this paper we highlight a strategy of split-intein mediated base-editor reconstitution for its adeno-associated virus (AAV) delivery. The purpose of this article is to offer readers with a better understanding of AAV-mediated base editors, and facilitate them to use this tool in in vivo experiments and potential clinical applications.
关键词	AAV delivery Base editors CRISPR Editing efficiency
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	通讯
WOS记录号	WOS:000697689700008
ESI学科分类	BIOLOGY & BIOCHEMISTRY
Scopus记录号	2-s2.0-85104093415
来源库	Scopus
引用统计	被引频次[WOS]：3
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/223766
专题	南方科技大学第一附属医院
作者单位	1.Shenzhen Eye Institute,Shenzhen Eye Hospital,Jinan University,Shenzhen,518000,China 2.Department of Ophthalmology,Shenzhen People's Hospital (The Second Clinical Medical College,Jinan University; The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,518020,China 3.School of Ophthalmology & Optometry,Shenzhen Eye Hospital,Shenzhen University,Shenzhen,518000,China 4.Institute of Microscale Optoelectronics,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen,518060,China
第一作者单位	南方科技大学第一附属医院
通讯作者单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Kuang，Jiajie,Lyu，Qinghua,Wang，Jiao,et al. Advances in base editing with an emphasis on an AAV-based strategy[J]. METHODS,2021.
APA	Kuang，Jiajie,Lyu，Qinghua,Wang，Jiao,Cui，Yubo,&Zhao，Jun.(2021).Advances in base editing with an emphasis on an AAV-based strategy.METHODS.
MLA	Kuang，Jiajie,et al."Advances in base editing with an emphasis on an AAV-based strategy".METHODS (2021).