Novel versatile 3D bio-scaffold made of natural biocompatible hagfish exudate for tissue growth and organoid modeling

Borzouyan Dastjerdi，Mahsa1; Amini，Abbas2,3; Nazari，Marziyeh4; Cheng，Chun5; Benson，Veronika6; Gholami，Ahmad7; Ghasemi，Younes8

2020-09-01

10.1016/j.ijbiomac.2020.05.024

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES   影响因子和分区

0141-8130

1879-0003

Hagfish exudate is a natural biological macromolecule made of keratin intermediate filament protein skeins and mucin vesicles. Here, we successfully examined this remarkable biomaterial as a substrate for three-dimensional (3D) cell culturing purposes. After the sterilization with chloroform vapor, Dulbecco's modified eagle medium was mixed with the exudate to rupture the vesicles and skeins; a highly soft, adherent, fibrous and biocompatible hydrogel was formed. A variety of cells, including Hela-FUCCI, NMuMG-FUCCI, 10T1/2 and C2C12, was cultured on the hagfish exudate. A remarkable 3D growth by ~2.5 folds after day 3, ~5 folds after day 5, ~10 folds after day 7 and ~15 folds after day 14 were seen compared to day one of culturing in the hagfish exudate scaffold. In addition, the phase contrast, fluorescent and confocal microscopy observations confirmed the organoid shape formation within the three-week culture. The viability of cells was almost 100% indicating the great in vitro and in vivo potential of this exceptional biomaterial with no cytotoxic effect.

Hagfish exudate Keratin intermediate filament scaffold Organoid Skein Slime hydrogel

SCI

英语

其他

WOS:000564487500004

BIOLOGY & BIOCHEMISTRY

2-s2.0-85084505762

Scopus

1.Department of Materials Science and Engineering,Graduate School of Engineering Science,Osaka University,Osaka,Japan
2.Department of Mechanical Engineering,Australian College of Kuwait,Safat,13015,Kuwait
3.Center for Infrastructure Engineering,Western Sydney University,Penrith,2751,Australia
4.Department of Mathematics and Physics,Australian College of Kuwait,Mishref,Kuwait
5.Department of Materials Science and Engineering,Southern University of Science and Technology (SUSTech),Shenzhen,518055,China
6.Institute of Microbiology,Czech Academy of Sciences,Videnska,Czech Republic
7.Pharmaceutical Sciences Research Center,Shiraz University of Medical Sciences,Shiraz,Iran
8.Biotechnology Research Center,Shiraz University of Medical Sciences,Shiraz,Iran

Borzouyan Dastjerdi，Mahsa,Amini，Abbas,Nazari，Marziyeh,et al. Novel versatile 3D bio-scaffold made of natural biocompatible hagfish exudate for tissue growth and organoid modeling[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,158:894-902.

Borzouyan Dastjerdi，Mahsa.,Amini，Abbas.,Nazari，Marziyeh.,Cheng，Chun.,Benson，Veronika.,...&Ghasemi，Younes.(2020).Novel versatile 3D bio-scaffold made of natural biocompatible hagfish exudate for tissue growth and organoid modeling.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,158,894-902.

Borzouyan Dastjerdi，Mahsa,et al."Novel versatile 3D bio-scaffold made of natural biocompatible hagfish exudate for tissue growth and organoid modeling".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 158(2020):894-902.