Heterochromatic Nonlinear Optical Responses in Upconversion Nanoparticles for Super-Resolution Nanoscopy

Chen, Chaohao1; Liu, Baolei1; Liu, Yongtao1; Liao, Jiayan1; Shan, Xuchen1; Wang, Fan1,2; Jin, Dayong1,3

2021-04-01

10.1002/adma.202008847

ADVANCED MATERIALS   影响因子和分区

0935-9648

1521-4095

Point spread function (PSF) engineering by an emitter's response can code higher-spatial-frequency information of an image for microscopy to achieve super-resolution. However, complexed excitation optics or repetitive scans are needed, which explains the issues of low speed, poor stability, and operational complexity associated with the current laser scanning microscopy approaches. Here, the diverse emission responses of upconversion nanoparticles (UCNPs) are reported for super-resolution nanoscopy to improve the imaging quality and speed. The method only needs a doughnut-shaped scanning excitation beam at an appropriate power density. By collecting the four-photon emission of single UCNPs, the high-frequency information of a super-resolution image can be resolved through the doughnut-emission PSF. Meanwhile, the two-photon state of the same nanoparticle is oversaturated, so that the complementary lower-frequency information of the super-resolution image can be simultaneously collected by the Gaussian-like emission PSF. This leads to a method of Fourier-domain heterochromatic fusion, which allows the extended capability of the engineered PSFs to cover both low- and high-frequency information to yield optimized image quality. This approach achieves a spatial resolution of 40 nm, 1/24th of the excitation wavelength. This work suggests a new scope for developing nonlinear multi-color emitting probes in super-resolution nanoscopy.

nonlinear optical responses PSF engineering super‐ resolution imaging upconversion nanoparticles

SCI ; EI

英语

NI论文

通讯

UTS Chancellor's Postdoctoral Research Fellowship[PRO186128] ; Australian Research Council (ARC) DECRA fellowship[DE200100074] ; ARC Discovery Project[DP190101058] ; National Natural Science Foundation of China (NSFC)[61729501] ; Major International (Regional) Joint Research Project of NSFC[51720105015] ; Science and Technology Innovation Commission of Shenzhen[KQTD20170810110913065] ; Australia China Science and Research Fund Joint Research Centre for Point-of-Care Testing["ACSRF658277","SQ2017YFGH001190"] ; China Scholarship Council[201607950009,201706020170,201607950010,201508530231,201708200004]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000640802200001

WILEY-V C H VERLAG GMBH

20211710243683

Image quality ; Laser excitation ; Nanoparticles ; Optical resolving power ; Optical transfer function ; Photons

Light/Optics:741.1 ; Nonlinear Optics:741.1.1 ; Laser Applications:744.9 ; Nanotechnology:761 ; Atomic and Molecular Physics:931.3 ; Solid State Physics:933

MATERIALS SCIENCE

Web of Science

1.Univ Technol Sydney, Fac Sci, Inst Biomed Mat & Devices IBMD, Sydney, NSW 2007, Australia
2.Univ Technol Sydney, Fac Engn & Informat Technol, Sch Elect & Data Engn, Sydney, NSW 2007, Australia
3.Southern Univ Sci & Technol, Dept Biomed Engn, UTS SUStech Joint Res Ctr Biomed Mat & Devices, Shenzhen 518055, Peoples R China

Chen, Chaohao,Liu, Baolei,Liu, Yongtao,et al. Heterochromatic Nonlinear Optical Responses in Upconversion Nanoparticles for Super-Resolution Nanoscopy[J]. ADVANCED MATERIALS,2021,33.

Chen, Chaohao.,Liu, Baolei.,Liu, Yongtao.,Liao, Jiayan.,Shan, Xuchen.,...&Jin, Dayong.(2021).Heterochromatic Nonlinear Optical Responses in Upconversion Nanoparticles for Super-Resolution Nanoscopy.ADVANCED MATERIALS,33.

Chen, Chaohao,et al."Heterochromatic Nonlinear Optical Responses in Upconversion Nanoparticles for Super-Resolution Nanoscopy".ADVANCED MATERIALS 33(2021).