Reversible Ratiometric NADH Sensing Using Semiconducting Polymer Dots

Chen, Haobin1; Yu, Jiangbo1; Men, Xiaoxiao2; Zhang, Jicheng1; Ding, Zhaoyang1; Jiang, Yifei1; Wu, Changfeng2; Chiu, Daniel T.1

2021-04-01

10.1002/anie.202100774

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   影响因子和分区

1433-7851

1521-3773

Reduced nicotinamide adenine dinucleotide (NADH) is a key coenzyme in living cells due to its role as an electron carrier in redox reactions, and its concentration is an important indicator of cell metabolic state. Abnormal NADH levels are associated with age-related metabolic diseases and neurodegenerative disorders, creating a demand for a simple, rapid analytical method for point-of-care NADH sensing. Here we develop a series of NADH-sensitive semiconducting polymer dots (Pdots) as nanoprobes for NADH measurement, and test their performance in vitro and in vivo. NADH sensing is based on electron transfer from semiconducting polymer chains in the Pdot to NADH upon UV excitation, quenching Pdot fluorescence emission. In polyfluorene-based Pdots, this mechanism resulted in an on-off NADH sensor; in DPA-CNPPV Pdots, UV excitation resulted in NADH-sensitive emission at two wavelengths, enabling ratiometric detection. Ratiometric NADH detection using DPA-CNPPV Pdots exhibits high sensitivity (3.1 mu M limit of detection), excellent selectivity versus other analytes, reversibility, and a fast response (less than 5 s). We demonstrate applications of the ratiometric NADH-sensing Pdots including smartphone-based NADH imaging for point-of-care use.

ratiometric sensor reduced nicotinamide adenine dinucleotide (NADH) semiconducting polymer dots (Pdots) smartphone biosensor

SCI ; EI

英语

NI论文

其他

National Institutes of Health[R01MH113333] ; Shenzhen Science and Technology Innovation Commission[KQTD20170810111314625] ; National Natural Science Foundation of China[81771930] ; National Key R&D Program of China["2020YFA0909000","2018YFB0407200"]

Chemistry

Chemistry, Multidisciplinary

WOS:000640659900001

WILEY-V C H VERLAG GMBH

20211610238384

Electron transport properties ; Metabolism ; Neurodegenerative diseases ; Redox reactions ; Smartphones

Medicine and Pharmacology:461.6 ; Telephone Systems and Equipment:718.1 ; Chemical Reactions:802.2 ; Polymeric Materials:815.1

CHEMISTRY

Web of Science

1.Univ Washington, Dept Chem & Bioengn, Seattle, WA 98195 USA
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 510855, Guangdong, Peoples R China

Chen, Haobin,Yu, Jiangbo,Men, Xiaoxiao,et al. Reversible Ratiometric NADH Sensing Using Semiconducting Polymer Dots[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2021,60:12007-12012.

Chen, Haobin.,Yu, Jiangbo.,Men, Xiaoxiao.,Zhang, Jicheng.,Ding, Zhaoyang.,...&Chiu, Daniel T..(2021).Reversible Ratiometric NADH Sensing Using Semiconducting Polymer Dots.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,60,12007-12012.

Chen, Haobin,et al."Reversible Ratiometric NADH Sensing Using Semiconducting Polymer Dots".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 60(2021):12007-12012.