microRNA-27b inhibits cell proliferation and invasion in bladder cancer by targeting engrailed-2

Li, Yunfei1; Duan, Qilin1; Gan, Lu1; Li, Wei2; Yang, Jianggen1; Huang, Guixiao1

2021

10.1042/BSR20201000

BIOSCIENCE REPORTS   影响因子和分区

0144-8463

1573-4935

["Background: Bladder cancer is considered a malignant tumour characterised by great heterogeneity. Engrailed-2 may be a gene implicated in bladder cancer. Bioinformatics analysis found base pair complementation between microRNA-27b and engrailed-2. The present study aimed to investigate the reciprocal association between microRNA-27b and engrailed-2 in bladder cancer.","Methods: The microRNA-27b and the protein of engrailed-2 in the tissues and cells of the bladder were detected. The processes of apoptosis, proliferation, invasion, and migration of tumour cells were evaluated. The co-action between microRNA-27b and engrailed-2 was detected by a luciferase reporter system. Finally, the interaction between microRNA-27b and engrailed-2 was further verified in vivo.","Results: The study found that the expression level of microRNA-27b is lower in bladder cancer tissues and cells than that in neighbouring ordinary tissues, whereas the opposite outcome was observed regarding the expression level of engrailed-2. Furthermore, microRNA-27b expression level is not significantly linked to the age of patients with bladder cancer; however, it is significantly associated with the clinicopathological grade of bladder cancer. Notably, engrailed-2 is negatively regulated by microRNA-27b. Transfection with microRNA-27b was associated with a significant reduction in the activity of bladder cancer cells and promoted apoptosis, while engrailed-2 restoration effectively reversed the above effects of microRNA-27b on bladder cancer in vitro and in vivo.","Conclusions: In conclusion, engrailed-2 is engaged in the development and process of bladder cancer through the negative mediation of microRNA-27b; additionally, microRNA-27b/engrailed-2 could form a signalling pathway with a significant effect on the process of bladder cancer."]

SCI

英语

其他

Basic Research Project of Shenzhen Science and Technology Plan[JCYJ20170307163313418] ; Sanming Project of Medicine in Shenzhen[SZSM201601043]

Biochemistry & Molecular Biology ; Cell Biology

Biochemistry & Molecular Biology ; Cell Biology

WOS:000629114800010

PORTLAND PRESS LTD

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Shenzhen Univ, Affilated Hosp 3, Dept Urol, Shenzhen 518000, Peoples R China
2.South Univ Sci & Technol China, Affiliated Hosp 1, Clin Med Res Ctr, Shenzhen 518020, Peoples R China

Li, Yunfei,Duan, Qilin,Gan, Lu,et al. microRNA-27b inhibits cell proliferation and invasion in bladder cancer by targeting engrailed-2[J]. BIOSCIENCE REPORTS,2021,41.

Li, Yunfei,Duan, Qilin,Gan, Lu,Li, Wei,Yang, Jianggen,&Huang, Guixiao.(2021).microRNA-27b inhibits cell proliferation and invasion in bladder cancer by targeting engrailed-2.BIOSCIENCE REPORTS,41.

Li, Yunfei,et al."microRNA-27b inhibits cell proliferation and invasion in bladder cancer by targeting engrailed-2".BIOSCIENCE REPORTS 41(2021).