Quantitative proteomics analysis of lysine 2-hydroxyisobutyrylation in IgA nephropathy

Huang, Shaoying1; Zheng, Fengping1; Lin, Hua3; Zhou, Xianqing3; Xu, Huixuan1; Zhang, Cantong1; Dai, Weier4; Hocher, Berthold5; Zhang, Xinzhou2; Tang, Donge1; Dai, Yong1,3

2021

10.1186/s12014-021-09314-0

Clinical Proteomics   影响因子和分区

1542-6416

1559-0275

Background Protein posttranslational modification is an indispensable regulatory element that can fine-tune protein functions and regulate diverse cellular processes. Lysine 2-hydroxyisobutyrylation (Khib) is a protein posttranslational modification that was recently identified and is thought to play a role in a wide variety of active cellular functions. Methods In this report, for the first time, we comparatively studied the 2-hydroxyisobutyrylation proteome in peripheral blood mononuclear cells from a biopsy-proven immunoglobulin A nephropathy (IgAN) group and a normal control group based on liquid chromatography-tandem mass spectrometry. Results Altogether, 7405 proteins were identified and added to a Khib library. Of these proteins, we identified 111 with upregulated expression and 83 with downregulated expression. Furthermore, we identified 428 Khib modification sites on 290 Khib-modified proteins, including 171 sites with increased modification on 122 Khib-modified proteins and 257 specific sites with reduced modification on 168 Khib-modified proteins. Conclusions Importantly, the abundance of lipocalin 2 was increased in the differentially expressed proteins, and a KEGG-based functional enrichment analysis showed that Khib proteins clustered in the IL-17 signaling pathway and phagosome category, which may have important associations with IgAN. Our data enlighten our understanding of Khib in IgAN and indicate that Khib may have important regulatory roles in IgAN.

Lysine 2-hydroxyisobutyrylation Mass spectrometry Immunoglobulin A nephropathy Peripheral blood mononuclear cells

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China[81671596] ; National Science Foundation for Young Scientists of China[31700795] ; science and technology plan of Shenzhen[JCYJ20190807153405508] ; Guangxi Key Laboratory of Metabolic Diseases Research[20-065-76] ; Shenzhen key Laboratory of Kindey Diseases[ZDSYS201504301616234]

Biochemistry & Molecular Biology

Biochemical Research Methods

WOS:000616315800001

BMC

Web of Science

1.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp,Guangdong Prov Engn Res Ctr, Affiliated Hosp 1,Clin Med Coll 2,Dept Clin Med R, Shenzhen 518020, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Jinan Univ, Affiliated Hosp 1, Clin Med Coll 2,Dept Nephrol,Shenzhen Peoples Hos, Shenzhen 518020, Peoples R China
3.Guilin 924 Hosp, Nephrol Dept, Guangxi Key Lab Metab Dis Res, Guilin 541002, Peoples R China
4.Univ Texas Austin, Coll Nat Sci, Austin, TX 78712 USA
5.Heidelberg Univ, Med Fac Mannheim, Dept Med Nephrol, Mannheim, Germany

Huang, Shaoying,Zheng, Fengping,Lin, Hua,et al. Quantitative proteomics analysis of lysine 2-hydroxyisobutyrylation in IgA nephropathy[J]. Clinical Proteomics,2021,18(1).

Huang, Shaoying.,Zheng, Fengping.,Lin, Hua.,Zhou, Xianqing.,Xu, Huixuan.,...&Dai, Yong.(2021).Quantitative proteomics analysis of lysine 2-hydroxyisobutyrylation in IgA nephropathy.Clinical Proteomics,18(1).

Huang, Shaoying,et al."Quantitative proteomics analysis of lysine 2-hydroxyisobutyrylation in IgA nephropathy".Clinical Proteomics 18.1(2021).