Urinary metabolomic analysis to identify potential markers for the diagnosis of tuberculosis and latent tuberculosis

Deng，Jiaheng1; Liu，Liguo1; Yang，Qianting2; Wei，Candong1; Zhang，Haoran1; Xin，Henan1; Pan，Shouguo3; Liu，Zisen3; Wang，Dakuan3; Liu，Bo1; Gao，Lei1; Liu，Rongmei4; Pang，Yu4; Chen，Xinchun5; Zheng，Jianhua1; Jin，Qi1

2021-06-15

10.1016/j.abb.2021.108876

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   影响因子和分区

0003-9861

1096-0384

Tuberculosis (TB) is a serious infectious disease with high infection and mortality rates. 5%–10% of the latent tuberculosis infections (LTBI) are likely to develop into active TB, and there are currently no clinical biomarkers that can distinguish between LTBI, active TB and other non-tuberculosis populations. Therefore, it is necessary to develop rapid diagnostic methods for active TB and LTBI. In this study, urinary metabolome of 30 active TB samples and the same number of LTBI and non-TB control samples were identified and analyzed by UPLC-Q Exactive MS. In total, 3744 metabolite components were obtained in ESI- mode and 4086 in ESI + mode. Orthogonal partial least square discriminant analysis (OPLS-DA) and hierarchical cluster analysis (HCA) showed that there were significant differences among LTBI, active TB and non-TB. Six differential metabolites were screened in positive and negative mode, 3-hexenoic acid, glutathione (GSH), glycochenodeoxycholate-3-sulfate, N-[4′-hydroxy-(E)-cinnamoyl]-L-aspartic acid, deoxyribose 5-phosphate and histamine. The overlapping pathways differential metabolites involved were mainly related to immune regulation and urea cycle. The results showed that the urine metabolism of TB patients was disordered and many metabolic pathways changed. Multivariate statistical analysis revealed that GSH and histamine were selected as potential molecular markers, with area under curve of receiver operating characteristic curve over 0.75. Among the multiple differential metabolites, GSH and histamine changed to varying degrees in active TB, LTBI and the non-TB control group. The levels of GSH and histamine in 48 urinary samples were measured by ELISA in validation phase, and the result in our study provided the potential for non-invasive biomarkers of TB.

Biomarkers Diagnostics Glutathione Histamine Metabolomics Tuberculosis Urine

SCI

英语

其他

WOS:000649260600003

BIOLOGY & BIOCHEMISTRY

2-s2.0-85104487097

Scopus

1.NHC Key Laboratory of Systems Biology of Pathogens,Institute of Pathogen Biology,and Center for Tuberculosis Research,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100730,China
2.National Clinical Research Center for Infectious Diseases,Guangdong Key Lab for Diagnosis & Treatment of Emerging Infectious Diseases,Shenzhen Third People's Hospital,Southern University of Science and Technology,Shenzhen,518112,China
3.Center for Diseases Control and Prevention of Zhongmu County,Zhongmu,451450,China
4.Department of Tuberculosis,Beijing Chest Hospital,Capital Medical University,Beijing Tuberculosis and Thoracic Tumor Research Institute,Beijing,No 97, Machang, Tongzhou District,101149,China
5.Department of Pathogen Biology,Shenzhen University School of Medicine,Shenzhen,518060,China

Deng，Jiaheng,Liu，Liguo,Yang，Qianting,et al. Urinary metabolomic analysis to identify potential markers for the diagnosis of tuberculosis and latent tuberculosis[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2021,704.

Deng，Jiaheng.,Liu，Liguo.,Yang，Qianting.,Wei，Candong.,Zhang，Haoran.,...&Jin，Qi.(2021).Urinary metabolomic analysis to identify potential markers for the diagnosis of tuberculosis and latent tuberculosis.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,704.

Deng，Jiaheng,et al."Urinary metabolomic analysis to identify potential markers for the diagnosis of tuberculosis and latent tuberculosis".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 704(2021).