A collection of toolkit strains reveals distinct localization and dynamics of membrane-associated transcripts in epithelia

Li，Zhimin1; Zhang，Pei1; Zhang，Ruotong1; Wang，Xinyan2; Tse，Yu Chung2; Zhang，Hongjie1

2021-05-04

10.1016/j.celrep.2021.109072

Cell Reports   影响因子和分区

2211-1247

Visualizing mRNA in real time in vivo at high resolution is critical for a full understanding of the spatiotemporal dynamics of gene regulation and function. Here, using a PP7/PCP-based mRNA-tagging approach, we construct a collection of tissue-specific and differentially expressed toolkit strains for visualizing mRNAs encoding apical, basolateral, and junctional proteins in Caenorhabditis elegans epithelia. We precisely delineate the spatiotemporal organization and dynamics of these transcripts across multiple subcellular compartments and tissues. Remarkably, all the transcripts exhibit an asymmetric, membrane-associated localization during epithelial polarization and maturation, which suggests that mRNA localization is a prerequisite for epithelial polarization and function. Single-particle tracking reveals striking features of the transport dynamics of the mRNAs in a gene-specific, compartment-linked, and time-resolved manner. The toolkit can be used to identify the cis-regulatory elements and trans-acting factors for mRNA localization. This study provides a valuable resource to investigate complex RNA dynamics in epithelial polarity and morphogenesis.

apical protein basolateral protein C. elegans epithelial polarity junctional component mRNA imaging mRNA localization

SCI

英语

其他

WOS:000647423700008

2-s2.0-85105112459

Scopus

1.Centre of Reproduction,Development and Aging,Faculty of Health Sciences,University of Macau,Taipa,999078,China
2.Core Research Facilities,Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China

Li，Zhimin,Zhang，Pei,Zhang，Ruotong,et al. A collection of toolkit strains reveals distinct localization and dynamics of membrane-associated transcripts in epithelia[J]. Cell Reports,2021,35(5).

Li，Zhimin,Zhang，Pei,Zhang，Ruotong,Wang，Xinyan,Tse，Yu Chung,&Zhang，Hongjie.(2021).A collection of toolkit strains reveals distinct localization and dynamics of membrane-associated transcripts in epithelia.Cell Reports,35(5).

Li，Zhimin,et al."A collection of toolkit strains reveals distinct localization and dynamics of membrane-associated transcripts in epithelia".Cell Reports 35.5(2021).