Extending the anion channelrhodopsin-based toolbox for plant optogenetics

Zhou，Yang1; Ding，Meiqi2; Duan，Xiaodong1,3; Konrad，Kai R.2; Nagel，Georg1; Gao，Shiqiang1

2021

10.3390/membranes11040287

Membranes   影响因子和分区

2077-0375

Optogenetics was developed in the field of neuroscience and is most commonly using light-sensitive rhodopsins to control the neural activities. Lately, we have expanded this technique into plant science by co-expression of a chloroplast-targeted β-carotene dioxygenase and an improved anion channelrhodopsin GtACR1 from the green alga Guillardia theta. The growth of Nicotiana tabacum pollen tube can then be manipulated by localized green light illumination. To extend the application of analogous optogenetic tools in the pollen tube system, we engineered another two ACRs, GtACR2, and ZipACR, which have different action spectra, light sensitivity and kinetic features, and characterized them in Xenopus laevis oocytes, Nicotiana benthamiana leaves and N. tabacum pollen tubes. We found that the similar molecular engineering method used to improve GtACR1 also enhanced GtACR2 and ZipACR performance in Xenopus laevis oocytes. The ZipACR1 performed in N. benthamiana mesophyll cells and N. tabacum pollen tubes with faster kinetics and reduced light sensitivity, allowing for optogenetic control of anion fluxes with better temporal resolution. The reduced light sensitivity would potentially facilitate future application in plants, grown under low ambient white light, combined with an optogenetic manipulation triggered by stronger green light.

Light-sensitive anion channel Optogenetics Pollen tube Rhodopsin Surface potential recording

SCI ; EI

英语

其他

Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)[374031971 TRR 240 A04] ; DFG[417451587,"KO 3657/2-3"]

Biochemistry & Molecular Biology ; Chemistry ; Engineering ; Materials Science ; Polymer Science

Biochemistry & Molecular Biology ; Chemistry, Physical ; Engineering, Chemical ; Materials Science, Multidisciplinary ; Polymer Science

WOS:000643239000001

MDPI

20211810305891

Ions ; Photosensitivity

Light/Optics:741.1

2-s2.0-85105070541

Scopus

1.Institute of Physiology,Department of Neurophysiology,Biocenter,University of Wuerzburg,Wuerzburg,97070,Germany
2.Institute for Molecular Plant Physiology and Biophysics,Julius-von-Sachs-Institute,University of Wuerzburg,Biocenter, Wuerzburg,97082,Germany
3.Department of Biology,College of Science,Southern University of Science and Technology (SUSTech),Shenzhen,518055,China

Zhou，Yang,Ding，Meiqi,Duan，Xiaodong,et al. Extending the anion channelrhodopsin-based toolbox for plant optogenetics[J]. Membranes,2021,11(4).

Zhou，Yang,Ding，Meiqi,Duan，Xiaodong,Konrad，Kai R.,Nagel，Georg,&Gao，Shiqiang.(2021).Extending the anion channelrhodopsin-based toolbox for plant optogenetics.Membranes,11(4).

Zhou，Yang,et al."Extending the anion channelrhodopsin-based toolbox for plant optogenetics".Membranes 11.4(2021).