Microscopic Afterglow Bioimaging by Ultralong Organic Phosphorescent Nanoparticles in Living Cells and Zebrafish

Zhou，Yudong1; Lu，Song1; Zhi，Jiahuan1; Jiang，Ruihong2; Chen，Jiahao3; Zhong，Hanbing3; Shi，Huifang1; Ma，Xing2; An，Zhongfu1

2021

10.1021/acs.analchem.1c00423

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Compared with short-lived emission probes featuring fluorescence imaging, the use of phosphorescent probes imparts the advantage of long-lived signal persistence that distinguishes against background fluorescence interference. However, the realization of ultralong organic phosphorescent (UOP) probes with an ultralong emission lifetime in an aqueous medium is still a challenge. Here, we present a rational strategy for obtaining UOP nanoparticles (NPs) in an air-saturated aqueous medium prepared using an organic phosphor (PDBCz) and a surfactant polymer (PVP), named PDBCz@PVP, showing an ultralong emission lifetime of 284.59 ms and a phosphorescence quantum efficiency of 7.6%. The excellent phosphorescence properties and water solubility of PDBCz@PVP make it a promising candidate for biological imaging. The as-prepared PDBCz@PVP NPs possess excellent luminescence intensity as well as illustrious biocompatibility both in vitro and in vivo. We demonstrate their use as an efficient phosphorescent nanoprobe both in living cells and zebrafish by capturing their afterglow emission signals under microscopy observation for the first time, realizing convenient and fast bioimaging with low cost, which allows for anti-fluorescence interference and shows promise for the future theragnostic applications in nanomedicine.

SCI ; EI

英语

NI论文

其他

National Natural Science Foundation of China[21975120,21875104] ; Shenzhen Science and Technology Program[KQTD20170809110344233] ; Shenzhen Bay Laboratory[SZBL201906281005]

Chemistry

Chemistry, Analytical

WOS:000645428400028

AMER CHEMICAL SOC

20211910309822

Biocompatibility ; Fluorescence imaging ; Medical nanotechnology ; Nanoparticles ; Probes ; Quantum efficiency

Immunology:461.9.1 ; Light/Optics:741.1 ; Nanotechnology:761 ; Quantum Theory; Quantum Mechanics:931.4 ; Solid State Physics:933

CHEMISTRY

2-s2.0-85105049477

Scopus

1.Key Laboratory of Flexible Electronics (KLOFE),Institute of Advanced Materials (IAM),Nanjing Tech University (NanjingTech),Nanjing,30 South Puzhu Road,211816,China
2.Sauvage Laboratory for Smart Materials,Shenzhen Bay Laboratory,Harbin Institute of Technology (Shenzhen),Shenzhen,No. 9 Duxue Road,518055,China
3.Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China

Zhou，Yudong,Lu，Song,Zhi，Jiahuan,et al. Microscopic Afterglow Bioimaging by Ultralong Organic Phosphorescent Nanoparticles in Living Cells and Zebrafish[J]. ANALYTICAL CHEMISTRY,2021,93(16):6516-6522.

Zhou，Yudong.,Lu，Song.,Zhi，Jiahuan.,Jiang，Ruihong.,Chen，Jiahao.,...&An，Zhongfu.(2021).Microscopic Afterglow Bioimaging by Ultralong Organic Phosphorescent Nanoparticles in Living Cells and Zebrafish.ANALYTICAL CHEMISTRY,93(16),6516-6522.

Zhou，Yudong,et al."Microscopic Afterglow Bioimaging by Ultralong Organic Phosphorescent Nanoparticles in Living Cells and Zebrafish".ANALYTICAL CHEMISTRY 93.16(2021):6516-6522.