Expanding of st11 carbapenemase-producing klebsiella pneumoniae subclones in a Chinese Hospital, Shenzhen, China

Zhang，Min1,2; Li，Jia3; Lu，Yuemei4; Wu，Weiyuan4; Wu，Jinsong4; Xu，Yanji1,5; Zhong，Yuxin1,2; Liu，Shiyi1,2; Lin，Chuchu6; Xu，Shuye3; Chen，Dandan1,2; Gao，Xuefeng7; Luo，Yutian8; Chen，Huaisheng8; Liu，Xueyan8; Huang，Wei1,2

2021

10.2147/IDR.S299478

Infection and Drug Resistance   影响因子和分区

1178-6973

Background: ST11 is the most prevalent sequence type of clinical Klebsiella pneumoniae in China. Methods: We investigated the characteristics of the ST11 subclones using core genome multi-locus sequence typing (cgMLST). Ninety-three carbapenemase-producing K. pneumoniae isolates were collected at Shenzhen People’s Hospital. Then, whole-genome sequencing and cgMLST were used to discriminate apparent subclones within the ST11 group. Results: We analyzed the prevalence and genetic relationships of these subclones. ST11 and K. pneumoniae carbapenemase (KPC-2) were the predominant genotype and carbapenemase, respectively, in the clinical carbapenemase-producing K. pneumoniae strains. cgMLST scheme genotyping divided the ST11 group into two clades across seven complex types (CTs). CT1313 was the most prevalent subclone. The deletion of galF and a high frequency of SNPs in genes associated with the stress-and SOS-responses were found in CT1291 and CT2405 over time, respectively. Conclusion: Our results indicated that the subclones of the ST11 group had different patterns of prevalence. Highly discriminatory genotyping techniques, such as cgMLST scheme, should be used in further molecular epidemiology investigations.

Carbapenem-resistance CgMLST Klebsiella pneumoniae ST11 Whole genome sequencing

SCI

英语

通讯

WOS:000641960000001

2-s2.0-85105013043

Scopus

1.Shenzhen People’s Hospital,Shenzhen Institute of Respiratory Diseases,Shenzhen, Guangdong,China
2.Bacteriology & Antibacterial Resistance Surveillance Laboratory,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University; The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen, Guangdong,China
3.Department of Nosocomial Infection Control,Bengbu Third People’s Hospital,Bengbu,China
4.Department of Clinical Microbiology,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University; The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen, Guangdong,China
5.Nanshan District Center of Chronic Disease Control,Shenzhen,China
6.Department of Nosocomial Infection Control,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University; The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen, Guangdong,China
7.Department of Gastroenterology and Hepatology,Shenzhen University General Hospital,Shenzhen,China
8.Department of Intensive Care Unit,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University; The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen, Guangdong,China

Zhang，Min,Li，Jia,Lu，Yuemei,et al. Expanding of st11 carbapenemase-producing klebsiella pneumoniae subclones in a Chinese Hospital, Shenzhen, China[J]. Infection and Drug Resistance,2021,14:1415-1422.

Zhang，Min.,Li，Jia.,Lu，Yuemei.,Wu，Weiyuan.,Wu，Jinsong.,...&Huang，Wei.(2021).Expanding of st11 carbapenemase-producing klebsiella pneumoniae subclones in a Chinese Hospital, Shenzhen, China.Infection and Drug Resistance,14,1415-1422.

Zhang，Min,et al."Expanding of st11 carbapenemase-producing klebsiella pneumoniae subclones in a Chinese Hospital, Shenzhen, China".Infection and Drug Resistance 14(2021):1415-1422.