Identification of a novel interplay between intestinal bacteria and metabolites in Chinese patients with IgA nephropathy via integrated microbiome and metabolome approaches

Wu, Hongwei1; Tang, Donge2; Zheng, Fengping2; Li, Shanshan2; Zhang, Xinzhou2; Yin, Lianghong1; Liu, Fanna1; Dai, Yong2

2021

10.21037/atm-20-2506

Annals of Translational Medicine   影响因子和分区

2305-5839

2305-5847

["Background: Immunoglobulin A nephropathy (IgAN) is the most common form of primary glomerulonephritis. The intestinal microbial ecosystem and metabolic network of IgAN have not been systematically analyzed. The present study aims to improve understanding of the gut microbiota and its metabolic capabilities to facilitate the development of diagnostic, therapeutic, and prognostic methods for IgAN.","Methods: We characterized the gut microbiota and metabolic patterns of fecal and serum samples of IgAN patients and healthy controls from the south of China using 16s ribosomal RNA gene sequencing and liquid chromatography-tandem mass spectrometry, respectively, and bioinformatics approaches.","Results: We found that the relative abundances of Streptococcus and Enterococcus were higher in IgAN patients, whereas Bacteroidetes and Bacteroides were lower. Changes in the gut microbiota of IgAN affected the metabolism and absorbance of microbiota-associated metabolites, in particular polyunsaturated fatty acids, free amino acid, and oligopeptides, and activated the phenylalanine metabolism pathway, thereby constructing a unique metabolic system of IgAN. We identified six pivotal metabolites, including bilirubin, trimethoprim, stearamide, phenylalanine, cis-9,10-epoxystearic acid, and phosphatidylethanolamine 17:0, that connected the metabolic networks of the gut and blood. Additionally, 5-hydroxyeicosatetraenoic acid and 5-hydroxy-6E,8Z,11Z-eicosatrienoic acid were shown to be associated with the classification of glomerular sclerosis.","Conclusions: We establish a relational network between microbiota, fecal metabolites, and serum metabolites in IgAN. The core microbiota and metabolites identified have promising value in therapeutic applications."]

Immunoglobulin A nephropathy (IgAN) microbiome metabolome metabolic network

SCI

英语

通讯

National Natural Science Foundation of China[81671596]

Oncology ; Research & Experimental Medicine

Oncology ; Medicine, Research & Experimental

WOS:000640889600013

AME PUBL CO

Web of Science

1.Jinan Univ, Affiliated Hosp 1, Dept Nephrol & Blood Purificat, Guangzhou, Peoples R China
2.Jinan Univ, Southern Univ Sci & Technol, Affiliated Hosp 1, Clin Med Coll 2,Shenzhen Peoples Hosp, Shenzhen, Peoples R China

Wu, Hongwei,Tang, Donge,Zheng, Fengping,et al. Identification of a novel interplay between intestinal bacteria and metabolites in Chinese patients with IgA nephropathy via integrated microbiome and metabolome approaches[J]. Annals of Translational Medicine,2021,9(1).

Wu, Hongwei.,Tang, Donge.,Zheng, Fengping.,Li, Shanshan.,Zhang, Xinzhou.,...&Dai, Yong.(2021).Identification of a novel interplay between intestinal bacteria and metabolites in Chinese patients with IgA nephropathy via integrated microbiome and metabolome approaches.Annals of Translational Medicine,9(1).

Wu, Hongwei,et al."Identification of a novel interplay between intestinal bacteria and metabolites in Chinese patients with IgA nephropathy via integrated microbiome and metabolome approaches".Annals of Translational Medicine 9.1(2021).