Rutin maintains redox balance to relieve oxidative stress induced by TBHP in nucleus pulposus cells

Zhou, Jian1; Liu, Qi2; Yang, Zhou3; Xie, Chuhai2; Ling, Long2; Hu, Hailan2; Cao, Yanming2; Huang, Yan2; Hua, Yue4

2021-04-01

10.1007/s11626-021-00581-7

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL   影响因子和分区

1071-2690

1543-706X

Rutin is well known for its anti-inflammatory and antioxidant properties against oxidative stress. However, its protective function in nucleus pulposus cells (NPCs) remains unclear. This study was aimed to explore the effects of rutin on oxidative stress in NPCs. Primary NPCs were obtained from 1-mo-old SD rats. The NPCs were treated with tert-butyl hydrogen peroxide (TBHP) to obtain the oxidative stress, and different concentrations of rutin were used to observe its influence on the oxidative stress in NPCs. Fluorescent probe DCFH-DA was used to detect reactive oxide species (ROS). The antioxidant proteins and genes of heat shock protein 70 (HSP70), manganese superoxide dismutase (Mn-SOD), catalase, aggrecan and collagen II in NPCs were measured by western blot and real-time PCR. With the stimulation of TBHP, the content of ROS in NPCs increased significantly and showed solubility correlation. Rutin effectively reduced the accumulation of ROS in a dose-dependent manner. The antioxidant proteins of HSP70, Mn-SOD, and catalase and the matrix proteins of aggrecan and collagen II decreased remarkably with the stimulation of TBHP, while the matrix metalloproteinase-13 (MMP-13) significantly increased after TBHP intervention. Rutin boosted the expressions of the HSP70, Mn-SOD, and catalase, elevated the contents of aggrecan and collagen II, and inhibited the expression of MMP-13 in NPCs. The findings of this study suggested that rutin is able to reverse oxidative stress and maintain cellular function of NPCs, and it was indicated that rutin could be a possible therapeutic option for intervertebral disc degeneration.

Rutin Intervertebral disc degeneration Oxidative stress Reactive oxide species

SCI

英语

其他

Scientific Research Projects of Administration of Traditional Chinese Medicine of Guangdong[20211243,20201231] ; Basic and Applied Basic Research Fund Project of Guangdong Province[2020A1515110631] ; Health Science and Technology Project of Guangzhou[20211A011078] ; Medical Science and Technology Research Fund Project of Guangdong[2020111220372206] ; Science and Technology Plan Project of Guangzhou[201704020032]

Cell Biology ; Developmental Biology

Cell Biology ; Developmental Biology

WOS:000645294300001

SPRINGER

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Nanchang Hongdu Hosp TCM, Dept Spine & Joint Surg, Nanchang, Jiangxi, Peoples R China
2.Guangzhou Med Univ, Dept Orthopaed Surg, Affiliated Hosp 2, 250 Chang Gang Dong Rd, Guangzhou 510260, Guangdong, Peoples R China
3.Southern Univ Sci & Technol Hosp, Dept Orthopaed Surg, Shenzhen, Peoples R China
4.Southern Med Univ, Sch Tradit Chinese Med, 1063 Shatai Rd, Guangzhou 510515, Guangdong, Peoples R China

Zhou, Jian,Liu, Qi,Yang, Zhou,et al. Rutin maintains redox balance to relieve oxidative stress induced by TBHP in nucleus pulposus cells[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2021.

Zhou, Jian.,Liu, Qi.,Yang, Zhou.,Xie, Chuhai.,Ling, Long.,...&Hua, Yue.(2021).Rutin maintains redox balance to relieve oxidative stress induced by TBHP in nucleus pulposus cells.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL.

Zhou, Jian,et al."Rutin maintains redox balance to relieve oxidative stress induced by TBHP in nucleus pulposus cells".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2021).