南方科技大学知识苑(SUSTech KC): SET knockdown attenuated phenotype modulation and calcium channel associated markers of airway smooth muscle cells in asthmatic mice

题名	SET knockdown attenuated phenotype modulation and calcium channel associated markers of airway smooth muscle cells in asthmatic mice
作者	Li, Jie ; He, Qi; Wang, Lingwei; Chen, Dandan; Qiu, Chen; Xu, Peng ; Lu, Yongzhen; Zeng, Yuwei; Chen, Rongchang
通讯作者	Li, Jie; Chen, Rongchang
发表日期	2021-04-01
DOI	10.21037/atm-21-573
发表期刊	Annals of Translational Medicine 影响因子和分区
ISSN	2305-5839
EISSN	2305-5847
卷号	9 期号:8
摘要	["Background: Dysfunctional phenotype modulation and calcium channels in airway smooth muscle cells (ASMCs) are important characteristics of airway remodeling in chronic asthma. However, the mechanisms underlying these pathological processes remain unclear. SET (I2PP2A, inhibitor-2 of protein phosphatase 2A) has many significant functions and is involved in various physiological and pathological processes. This study aimed to determine the function of SET in chronic asthma.","Methods: BALB/c mice were sensitized by ovalbumin injection and repeated inhalation of ovalbumin. The Penh value was measured using the Buxco whole body plethysmography system. A short hairpin RNA of the SET gene was designed and transfected into ASMCs derived from asthmatic mice. Flow cytometry of Annexin-V/propidium iodide staining was used for evaluating cell apoptosis. Western blot was adopted to measure the expression levels of ASMCs phenotype modulation markers and calcium channel-associated proteins.","Results: The results showed that shRNA targeting SET significantly decreased the expression of SET, and enhanced the apoptosis of ASMCs. SET knockdown promoted the expression of contractile phenotype markers such as alpha-SMA (alpha smooth muscle Actin), SM-MHC (smooth muscle Myosin heavy chain), and calponin, and inhibited the expression of synthetic phenotype markers including vimentin and CD44. The expression of the calcium channel-related proteins STIM1 (Stromal interaction molecule 1) and Orai1 were also inhibited after SET knockdown.","Conclusions: These data demonstrated that SET participated in the development of airway dysfunction in asthma, suggesting that the silencing of SET may be a new therapeutic target for the treatment of asthma patients."]
关键词	Asthma airway smooth muscle cells (ASMCs) SET phenotype modulation calcium channel
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	NSFC[81770028]
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS类目	Oncology ; Medicine, Research & Experimental
WOS记录号	WOS:000647716600011
出版者	AME PUBL CO
来源库	Web of Science
引用统计	被引频次[WOS]：1
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/228395
专题	南方科技大学第一附属医院
作者单位	Jinan Univ, Southern Univ Sci & Technol, Clin Med Coll 2,Shenzhen Inst Resp Dis, Affiliated Hosp 1,Shenzhen Peoples Hosp,Key Lab S, Shenzhen, Peoples R China
第一作者单位	南方科技大学第一附属医院
通讯作者单位	南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Li, Jie,He, Qi,Wang, Lingwei,et al. SET knockdown attenuated phenotype modulation and calcium channel associated markers of airway smooth muscle cells in asthmatic mice[J]. Annals of Translational Medicine,2021,9(8).
APA	Li, Jie.,He, Qi.,Wang, Lingwei.,Chen, Dandan.,Qiu, Chen.,...&Chen, Rongchang.(2021).SET knockdown attenuated phenotype modulation and calcium channel associated markers of airway smooth muscle cells in asthmatic mice.Annals of Translational Medicine,9(8).
MLA	Li, Jie,et al."SET knockdown attenuated phenotype modulation and calcium channel associated markers of airway smooth muscle cells in asthmatic mice".Annals of Translational Medicine 9.8(2021).