Expansion Microscopy with Multifunctional Polymer Dots

Liu, Jie1,2; Fang, Xiaofeng1; Liu, Zhihe1; Li, Rongqin3; Yang, Yicheng1; Sun, Yujie3; Zhao, Zhongying2; Wu, Changfeng1

2021-05-01

10.1002/adma.202007854

ADVANCED MATERIALS   影响因子和分区

0935-9648

1521-4095

Expansion microscopy (ExM) provides nanoscale resolution on conventional microscopes via physically enlarging specimens with swellable polyelectrolyte gels. However, challenges involving fluorophore degradation and dilution during sample expansion have yet to be overcome. Herein, sequential cellular targeting, gel anchoring, and high-fidelity fluorescence reported using multifunctional polymer dots (Pdots) designed for ExM applications are demonstrated. The impressive brightness of the Pdots facilitates multicolor ExM, thereby enabling visualization of a variety of subcellular structures and neuron synapses. The average fluorescence intensities of Pdots in ExM range from approximate to 3 to 6 times higher than those achieved using commercially available Alexa dyes. Moreover, the fluorescence brightness and optical fluctuation are significantly improved by a surfactant-containing expansion buffer, which enables further resolution enhancement via super-resolution optical fluctuation imaging (SOFI). The combination of ExM and SOFI allows subcellular structures of approximate to 30 nm to be resolved by conventional microscopes. These results highlight the immense potential of multifunctional Pdots for ExM-enhanced super-resolution imaging.

expansion microscopy fluorescent probes polymer dots subcellular labeling super‐ resolution optical fluctuation microscopy

SCI ; EI

英语

NI论文

第一 ; 通讯

National Key R&D Program of China["2020YFA0909000","2018YFB0407200"] ; Shenzhen Science and Technology Innovation Commission[KQTD20170810111314625] ; National Natural Science Foundation of China[81771930]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000650228700001

WILEY-V C H VERLAG GMBH

20212010357171

Fluorescence ; Luminance ; Optical resolving power ; Polyelectrolytes

Light/Optics:741.1 ; Organic Polymers:815.1.1 ; Materials Science:951

MATERIALS SCIENCE

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Hong Kong Baptist Univ, Dept Biol, Hong Kong 999077, Peoples R China
3.Peking Univ, Sch Life Sci, State Key Lab Membrane Biol Biomed Pioneer Innova, Beijing 100871, Peoples R China

Liu, Jie,Fang, Xiaofeng,Liu, Zhihe,et al. Expansion Microscopy with Multifunctional Polymer Dots[J]. ADVANCED MATERIALS,2021,33.

Liu, Jie.,Fang, Xiaofeng.,Liu, Zhihe.,Li, Rongqin.,Yang, Yicheng.,...&Wu, Changfeng.(2021).Expansion Microscopy with Multifunctional Polymer Dots.ADVANCED MATERIALS,33.

Liu, Jie,et al."Expansion Microscopy with Multifunctional Polymer Dots".ADVANCED MATERIALS 33(2021).