南方科技大学知识苑(SUSTech KC): The chromatin accessibility landscape reveals distinct transcriptional regulation in the induction of human primordial germ cell-like cells from pluripotent stem cells

题名	The chromatin accessibility landscape reveals distinct transcriptional regulation in the induction of human primordial germ cell-like cells from pluripotent stem cells
作者	Wang，Xiaoman 1,3; Veerapandian，Veeramohan 2,3; Yang，Xinyan 3; Song，Ke 3; Xu，Xiaoheng 3; Cui，Manman 3; Yuan，Weiyan 3; Huang，Yaping 3; Xia，Xinyu 3; Yao，Zhaokai 3; Wan，Cong 3; Luo，Fang 3; Song，Xiuling 3; Wang，Xiaoru 3; Zheng，Yi 3; Hutchins，Andrew Paul6 ; Jauch，Ralf 7; Liang，Meiyan 2; Wang，Chenhong 1; Liu，Zhaoting 3; Chang，Gang 4; Zhao，Xiao Yang 3,5,8,9,10,11
通讯作者	Liu，Zhaoting
发表日期	2021-05-11
DOI	10.1016/j.stemcr.2021.03.032
发表期刊	Stem Cell Reports 影响因子和分区
ISSN	2213-6711
卷号	16 期号:5页码:1245-1261
摘要	In vitro induction of human primordial germ cell-like cells (hPGCLCs) provides an ideal platform to recapitulate hPGC development. However, the detailed molecular mechanisms regulating the induction of hPGCLCs remain largely uncharacterized. Here, we profiled the chromatin accessibility and transcriptome dynamics throughout the process of hPGCLC induction. Genetic ablation of SOX15 indicated the crucial roles of SOX15 in the maintenance of hPGCLCs. Mechanistically, SOX15 exerted its roles via suppressing somatic gene expression and sustaining latent pluripotency. Notably, ETV5, a downstream regulator of SOX15, was also uncovered to be essential for hPGCLC maintenance. Finally, a stepwise switch of OCT4/SOX2, OCT4/SOX17, and OCT4/SOX15 binding motifs were found to be enriched in closed-to-open regions of human embryonic stem cells, and early- and late-stage hPGCLCs, respectively. Collectively, our data characterized the chromatin accessibility and transcriptome landscapes throughout hPGCLC induction and defined the SOX15-mediated regulatory networks underlying this process.
关键词	Chromatin Accessibility Etv5 Germ Cell Human Primordial Germ Cell-like Cells Sox15
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	其他
WOS记录号	WOS:000649800300006
Scopus记录号	2-s2.0-85105253855
来源库	Scopus
引用统计	被引频次[WOS]：17
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/228437
专题	生命科学学院_生物系生命科学学院
作者单位	1.Shenzhen Hospital of Southern Medical University,Shenzhen,China 2.Shunde Hospital of Southern Medical University,Shunde,China 3.Department of Developmental Biology,School of Basic Medical Sciences,Southern Medical University,Guangzhou,China 4.Guangdong Provincial Key Laboratory of Regional Immunity and Diseases,Department of Biochemistry and Molecular Biology,Shenzhen University Health Science Center,Shenzhen,China 5.Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory),Guangzhou,China 6.Department of Biology,Southern University of Science and Technology,Shenzhen,China 7.School of Biomedical Sciences,Li Ka Shing Faculty of Medicine,The University of Hong Kong,Hong Kong SAR,Hong Kong 8.State Key Laboratory of Organ Failure Research,Department of Developmental Biology,School of Basic Medical Sciences,Southern Medical University,Guangzhou,China 9.Key Laboratory of Mental Health of the Ministry of Education,Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence,Southern Medical University,Guangzhou,China 10.Department of Obstetrics and Gynecology,Zhujiang Hospital,Southern Medical University,Guangzhou,China 11.National Clinical Research Center for Kidney Disease,Guangzhou,China
推荐引用方式 GB/T 7714	Wang，Xiaoman,Veerapandian，Veeramohan,Yang，Xinyan,et al. The chromatin accessibility landscape reveals distinct transcriptional regulation in the induction of human primordial germ cell-like cells from pluripotent stem cells[J]. Stem Cell Reports,2021,16(5):1245-1261.
APA	Wang，Xiaoman.,Veerapandian，Veeramohan.,Yang，Xinyan.,Song，Ke.,Xu，Xiaoheng.,...&Zhao，Xiao Yang.(2021).The chromatin accessibility landscape reveals distinct transcriptional regulation in the induction of human primordial germ cell-like cells from pluripotent stem cells.Stem Cell Reports,16(5),1245-1261.
MLA	Wang，Xiaoman,et al."The chromatin accessibility landscape reveals distinct transcriptional regulation in the induction of human primordial germ cell-like cells from pluripotent stem cells".Stem Cell Reports 16.5(2021):1245-1261.