Andrographolide Against Lung Cancer-New Pharmacological Insights Based on High-Throughput Metabolomics Analysis Combined with Network Pharmacology

Luo，Wen1,2; Jia，Li2; Zhang，Jia Wen2; Wang，Dong Jie2; Ren，Qiu3; Zhang，Wei2

2021-04-21

10.3389/fphar.2021.596652

Frontiers in Pharmacology   影响因子和分区

1663-9812

Andrographolide (Andro) has known to treat various illnesses such as colds, diarrhea, fever and infectious diseases. However, the effect mechanism of Andro is still unclear. Therefore, we used high-throughput metabolomics analysis to discover biomarkers, metabolic profiles and pathways to reveal the pharmacological action and effective mechanism of Andro against lung cancer. The metabolic effects of Andro on lung cancer animal was explored by ultra-performance liquid chromatography-triple-time of flight/mass spectrometry (UPLC-TOF/MS) analysis. Our results showed that Andro exhibited significant protective effects against lung cancer. Compared with control group, a total of 25 metabolites biomarkers was identified in urine of model animals, which 18 of them were regulated toward the normal direction after Andro treatment, and network pharmacology analysis showed that they were related with 570 proteins. Biological pathways analysis showed that the 11 metabolism pathways were regulated by Andro treatment in lung cancer mouse, and amino acid metabolism and arachidonic acid metabolism have great potential as target pathways for Andro against lung cancer. It revealed that high-throughput metabolomics combined with network pharmacology analysis provides deeply insight into the therapeutic mechanisms of natural product for promoting medicine development and disease treatment.

liquid chromatography lung cancer metabolic pathway target untargeted metabolomics urine biomarker

SCI

英语

第一

WOS:000647014700001

2-s2.0-85105276021

Scopus

1.Respiratory Department,National Clinical Research Center for Infectious Disease,Shenzhen Third People's Hospital,The Second Affiliated Hospital,School of Medicine,Southern University of Science and Technology,Shenzhen,China
2.Department of Respiratory and Critical Care,First Affiliated Hospital,Harbin Medical University,Harbin,China
3.Department of Respiratory Medicine,Heilongjiang Provincial Hospital,Harbin,China

Luo，Wen,Jia，Li,Zhang，Jia Wen,et al. Andrographolide Against Lung Cancer-New Pharmacological Insights Based on High-Throughput Metabolomics Analysis Combined with Network Pharmacology[J]. Frontiers in Pharmacology,2021,12.

Luo，Wen,Jia，Li,Zhang，Jia Wen,Wang，Dong Jie,Ren，Qiu,&Zhang，Wei.(2021).Andrographolide Against Lung Cancer-New Pharmacological Insights Based on High-Throughput Metabolomics Analysis Combined with Network Pharmacology.Frontiers in Pharmacology,12.

Luo，Wen,et al."Andrographolide Against Lung Cancer-New Pharmacological Insights Based on High-Throughput Metabolomics Analysis Combined with Network Pharmacology".Frontiers in Pharmacology 12(2021).