Characteristics of biofilm-induced degradation at resin–dentin interfaces using multiple combinations of adhesives and resins

Zhou，Yuan1; Matin，Khairul2,3; Shimada，Yasushi2,4; Sadr，Alireza5; Wang，Guoqing6; Tagami，Junji2; Feng，Xiping1

2021

10.1016/j.dental.2021.04.007

DENTAL MATERIALS   影响因子和分区

0109-5641

Objective: We aimed to evaluate morphological, mechanical and chemical characteristics at resin–dentin interfaces using multiple combinations of adhesives and resins after a short-term biofilm-induced degradation. Methods: Cervical cavities were prepared in bovine incisors, treated by Clearfil SE Bond 2 (SE) or FL-Bond II (FL), restored by Clearfil Majesty ES Flow (ES) or Beautifil Flow Plus (BFP) and grouped into SE-ES, SE-BFP, FL-ES and FL-BFP. After biofilm challenge, interfacial gaps and dentin wall lesions were examined by optical coherence tomography (OCT). Gap depth (GD), gap pattern scale (GPS) and dentin wall lesion depth (WLD) were evaluated from confocal laser scanning microscope. Microhardness of dentin lesions was measured with a Vickers microhardness tester. Chemical elements in resins and dentin wall lesions were analyzed by scanning electron microscopy and energy dispersive X-ray spectrometry (SEM/EDS). Morphological structures of interfacial gaps were observed by SEM. Results: OCT could detect adhesive-dentin-bonded and adhesive-dentin-debonded gaps. SE-containing groups showed significantly lower GPS than FL-containing groups. FL-BFP showed significantly lower WLD than FL-ES. Microhardness of dentin wall lesions was higher than that of outer lesions and they showed significant differences in FL-BFP. SE-BFP showed a lower GPS curve and higher intensities of Ca and P in the upper half of dentin wall lesions than other groups. From SEM, microgaps between filler and matrix, break and loss of matrix, separation of adhesive matrix with hybrid layer occurred at interfacial gaps. Significance: The morphological, mechanical and chemical characteristics of resin–dentin interfacial degradation depend on the component and chemistry of restorative materials.

Adhesive Cariogenic biofilms CLSM Composite resin Dentin wall lesions Interfacial gaps Microhardness Optical coherence tomography (OCT) SEM/EDS

SCI ; EI

英语

其他

WOS:000675411800009

20211910320642

Biofilms ; Chemical analysis ; Chemical elements ; Degradation ; Mammals ; Microhardness ; Optical tomography ; Resins ; Scanning electron microscopy

Biomaterials (including synthetics):462.5 ; Optical Devices and Systems:741.3 ; Chemical Reactions:802.2 ; Chemical Products Generally:804 ; Organic Polymers:815.1.1 ; Materials Science:951

CLINICAL MEDICINE

2-s2.0-85105283165

Scopus

1.Laboratory of Molecular and Preventive Dentistry,Department of Preventive Dentistry,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,639 Zhizaoju Road, Huangpu District,200011,China
2.Department of Cariology and Operative Dentistry,Graduate School of Medical and Dental Sciences,Tokyo Medical and Dental University,Tokyo,1-5-45 Yushima, Bunkyo-ku,113-8549,Japan
3.Endowed Department of International Oral Health Science (affiliated with Department of Translational Research),School of Dental Medicine,Tsurumi University,Yokohama-shi,2-1-3 Tsurumi, Tsurumi-ku,230-0063,Japan
4.Department of Operative Dentistry,Graduate School of Medicine,Dentistry and Pharmaceutical Sciences,Okayama University,Okayama,2-5-1 Shikata-cho, Kita-ku,700-8525,Japan
5.Biomimetics Biomaterials Biophotonics & Technology Laboratory,Department of Restorative Dentistry,University of Washington School of Dentistry,Seattle,1959 NE Pacific Street,98195-7456,United States
6.Department of Electrical and Electronic Engineering,Southern University of Science and Technology,Shenzhen,1088 Xueyuan Ave, Nanshan District,518055,China

Zhou，Yuan,Matin，Khairul,Shimada，Yasushi,等. Characteristics of biofilm-induced degradation at resin–dentin interfaces using multiple combinations of adhesives and resins[J]. DENTAL MATERIALS,2021,37:1260-1272.

Zhou，Yuan.,Matin，Khairul.,Shimada，Yasushi.,Sadr，Alireza.,Wang，Guoqing.,...&Feng，Xiping.(2021).Characteristics of biofilm-induced degradation at resin–dentin interfaces using multiple combinations of adhesives and resins.DENTAL MATERIALS,37,1260-1272.

Zhou，Yuan,et al."Characteristics of biofilm-induced degradation at resin–dentin interfaces using multiple combinations of adhesives and resins".DENTAL MATERIALS 37(2021):1260-1272.