Nanomechanics of Lignin-Cellulase Interactions in Aqueous Solutions

Zheng, Peitao1,2; Xiang, Li2; Chang, Jian3; Lin, Qiaojia1; Xie, Lei2; Lan, Tu2,4; Liu, Jing1; Gong, Zhenggang1; Tang, Tian4; Shuai, Li1; Luo, Xiaolin1,5; Chen, Nairong1; Zeng, Hongbo2

2021-05-10

10.1021/acs.biomac.1c00140

BIOMACROMOLECULES   影响因子和分区

1525-7797

1526-4602

Efficient enzymatic hydrolysis of cellulose in lignocellulose to glucose is one of the most critical steps for the production of biofuels. The nonproductive adsorption of lignin to expensive cellulase highly impedes the development of biorefinery. Understanding the lignin-cellulase interaction mechanism serves as a vital basis for reducing such nonproductive adsorption in their practical applications. Yet, limited report is available on the direct characterization of the lignin-cellulase interactions. Herein, for the first time, the nanomechanics of the biomacromolecules including lignin, cellulase, and cellulose were systematically investigated by using a surface force apparatus (SFA) at the nanoscale in aqueous solutions. Interestingly, a cation-pi interaction was discovered and demonstrated between lignin and cellulase molecules through SFA measurements with the addition of different cations (Na+, K+, etc.). The complementary adsorption tests and theoretical calculations further confirmed the validity of the force measurement results. This finding further inspired the investigation of the interaction between lignin and other noncatalytic-hydrolysis protein (i.e., soy protein). Soy protein was demonstrated as an effective, biocompatible, and inexpensive lignin-blocker based on the molecular force measurements through the combined effects of electrostatic, cation-pi, and hydrophobic interactions, which significantly improved the enzymatic hydrolysis efficiencies of cellulose in pretreated lignocellulosic substrates. Our results offer quantitative information on the fundamental understanding of the lignin-cellulase interaction mechanism. Such unraveled nanomechanics provides new insights into the development of advanced biotechnologies for addressing the nonproductive adsorption of lignin to cellulase, with great implications on improving the economics of lignocellulosic biorefinery.

SCI ; EI

英语

其他

National Natural Science Foundation of China[31870559,31901262] ; Fujian Provincial Department of Science and Technology["2018J01590","2019J01387"] ; Finance Department of Fujian Province[K81600001] ; Jiangsu Provincial Key Laboratory of Pulp and Paper Science and Technology[KL201911]

Biochemistry & Molecular Biology ; Chemistry ; Polymer Science

Biochemistry & Molecular Biology ; Chemistry, Organic ; Polymer Science

WOS:000651049600023

AMER CHEMICAL SOC

20212210425240

Adsorption ; Biocompatibility ; Cellulose derivatives ; Enzymatic hydrolysis ; Force measurement ; Hydrophobicity ; Lignin ; Nanomechanics ; Positive ions ; Refining

Immunology:461.9.1 ; Nanotechnology:761 ; Chemical Reactions:802.2 ; Chemical Operations:802.3 ; Cellulose, Lignin and Derivatives:811.3 ; Physical Properties of Gases, Liquids and Solids:931.2 ; Solid State Physics:933 ; Mechanical Variables Measurements:943.2

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Fujian Agr & Forestry Univ, Coll Mat Engn, Fuzhou 350002, Fujian, Peoples R China
2.Univ Alberta, Dept Chem & Mat Engn, Edmonton, AB T6G 2V4, Canada
3.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Peoples R China
4.Univ Alberta, Dept Mech Engn, Edmonton, AB T6G 1H9, Canada
5.Nanjing Forestry Univ, Jiangsu Prov Key Lab Pulp & Paper Sci & Technol, Nanjing 210037, Jiangsu, Peoples R China

Zheng, Peitao,Xiang, Li,Chang, Jian,et al. Nanomechanics of Lignin-Cellulase Interactions in Aqueous Solutions[J]. BIOMACROMOLECULES,2021,22(5):2033-2042.

Zheng, Peitao.,Xiang, Li.,Chang, Jian.,Lin, Qiaojia.,Xie, Lei.,...&Zeng, Hongbo.(2021).Nanomechanics of Lignin-Cellulase Interactions in Aqueous Solutions.BIOMACROMOLECULES,22(5),2033-2042.

Zheng, Peitao,et al."Nanomechanics of Lignin-Cellulase Interactions in Aqueous Solutions".BIOMACROMOLECULES 22.5(2021):2033-2042.