Urinary proteomic analysis to identify a potential protein biomarker panel for the diagnosis of tuberculosis

Liu, Liguo1; Deng, Jiaheng1; Yang, Qianting2; Wei, Candong1; Liu, Bo1; Zhang, Haoran1; Xin, Henan1; Pan, Shouguo3; Liu, Zisen3; Wang, Dakuan3; Pang, Yu4; Chen, Xinchun5; Gao, Lei1; Zheng, Jianhua1; Liu, Rongmei4; Jin, Qi1

2021-06-01

10.1002/iub.2509

IUBMB LIFE   影响因子和分区

1521-6543

1521-6551

Tuberculosis (TB) is caused by Mycobacterium tuberculosis and is one of the primary causes of death worldwide. Rapid and accurate diagnosis of TB is one of the most direct means to reduce the incidence of TB. In this study, urinary proteomic profiling of TB patients and non-TB individual controls (HCs) was performed, and differentially expressed urinary proteins between TB and HCs were compared and exclusively expressed proteins in TB patients were selected to establish a clinically useful disease marker panel. In total, these top 11 targeted proteins with 265 peptides were scheduled for multiple reaction monitoring validation analysis by using urine samples from 52 TB patients and 52 HCs. The result demonstrated that a three-protein combination out of the five-protein panel (namely P22352, Q9P121, P15151, Q13291, and Q8NDA2) exhibited sensitivity rate of 82.7% in the diagnosis of TB. Furthermore, the three-protein combination could differentiate TB from the latent tuberculosis (LTB) effectively, which exhibited specificity rate of 92.3% for the diagnosis of TB from the LTB category. Although more numbers of clinical samples are required for further verification, the results provided preliminary evidence that this "three-protein combination" out of the five-protein panel could probably be a novel TB diagnostic biomarker in clinical application.

diagnostic biomarker multiple reaction monitoring tuberculosis ultra-performance liquid chromatography urinary proteomics

SCI

英语

其他

CAMS Innovation Fund for Medical Sciences[2016-I2M-1-013] ; Jin Qi team of Sanming Project of Medicine in Shenzhen[SZSM201412001] ; National Science and Technology Infrastructure of China (National Pathogen Resource Center)[NPRC-32] ; National Science and Technology Major Project of China[2017ZX10201301-002-003] ; Chinese Academy of Medical Sciences[2020-PT310-004] ; Sanming Project of Medicine in Shenzhen[GCZX2015043015340574]

Biochemistry & Molecular Biology ; Cell Biology

Biochemistry & Molecular Biology ; Cell Biology

WOS:000657838700001

WILEY

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Chinese Acad Med Sci & Peking Union Med Coll, NHC Key Lab Syst Biol Pathogens, Inst Pathogen Biol, Ctr TB Res, Beijing 100730, Peoples R China
2.Southern Univ Sci & Technol, Natl Clin Res Ctr Infect Dis, Guangdong Key Lab Diag & Treatment Emerging Infec, Shenzhen Third Peoples Hosp, Shenzhen, Peoples R China
3.Ctr Dis Control & Prevent Zhongmu Cty, Zhongmu, Peoples R China
4.Capital Med Univ, Beijing TB & Thorac Tumor Res Inst, Beijing Chest Hosp, Dept TB, 97 Machang, Beijing 101149, Peoples R China
5.Shenzhen Univ, Dept Pathogen Biol, Sch Med, Shenzhen, Peoples R China

Liu, Liguo,Deng, Jiaheng,Yang, Qianting,et al. Urinary proteomic analysis to identify a potential protein biomarker panel for the diagnosis of tuberculosis[J]. IUBMB LIFE,2021.

Liu, Liguo.,Deng, Jiaheng.,Yang, Qianting.,Wei, Candong.,Liu, Bo.,...&Jin, Qi.(2021).Urinary proteomic analysis to identify a potential protein biomarker panel for the diagnosis of tuberculosis.IUBMB LIFE.

Liu, Liguo,et al."Urinary proteomic analysis to identify a potential protein biomarker panel for the diagnosis of tuberculosis".IUBMB LIFE (2021).