Lif Deficiency Leads to Iron Transportation Dysfunction in Ameloblasts

Fan，L.1; Ou，Y. J.2; Zhu，Y. X.1; Liang，Y. D.3; Zhou，Y.1,4; Wang，Y. N.1,4

2021

10.1177/00220345211011986

JOURNAL OF DENTAL RESEARCH   影响因子和分区

0022-0345

1544-0591

Leukemia inhibitory factor (LIF), a member of the interleukin 6 family of cytokines, is involved in skeletal metabolism, blastocyst implantation, and stem cell pluripotency maintenance. However, the role of LIF in tooth development needs to be elucidated. The aim of the present study was to investigate the effect of Lif deficiency on tooth development and to elucidate the functions of Lif during tooth development and the underlying mechanisms. First, it was found that the incisors of Lif-knockout mice had a much whiter color than those of wild-type mice. Although there were no structural abnormalities or defective mineralization according to scanning electronic microscopy and computed tomography analysis, 3-dimensional images showed that the length of incisors was shorter in Lif mice. Microhardness and acid resistance assays showed that the hardness and acid resistance of the enamel surface of Lif mice were decreased compared to those of wild-type mice. In Lif mice, whose general iron status was comparable to that of the control mice, the iron content of the incisors was significantly reduced, as confirmed by energy-dispersive X-ray spectroscopy (EDS) and Prussian blue staining. Histological staining showed that the cell length of maturation-stage ameloblasts was shorter in Lif mice. Likewise, decreased expression of Tfrc and Slc40a1, both of which are crucial proteins for iron transportation, was observed in Lif mice and Lif-knockdown ameloblast lineage cell lines, according to quantitative reverse transcription polymerase chain reaction, immunohistochemistry, and Western blot. Moreover, the upregulation of Tfrc and Slc40a1 induced by Lif stimulation was blocked by Stattic, a signal transducer and activator of transcription 3 (Stat3) signaling inhibitor. These results suggest that Lif deficiency inhibits iron transportation in the maturation-stage ameloblasts, and Lif modulates expression of Tfrc and Slc40a1 through the Stat3 signaling pathway during enamel development.

amelogenesis dental enamel gene expression incisor knockout mice pigmentation

SCI

英语

其他

National Natural Science Foundation of China[82071090] ; Shenzhen Scientific Research Project[JCYJ20180302144621755]

Dentistry, Oral Surgery & Medicine

Dentistry, Oral Surgery & Medicine

WOS:000681419600001

SAGE PUBLICATIONS INC

CLINICAL MEDICINE

2-s2.0-85106562169

Scopus

1.The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education,School and Hospital of Stomatology,Wuhan University,Wuhan,China
2.Fujian Key Laboratory of Oral Diseases Fujian Provincial Engineering Research Center of Oral Biomaterial Stomatological Key Lab of Fujian College and University,School and Hospital of Stomatology,Fujian Medical University,Fuzhou,China
3.Yantian Hospital,Southern University of Science and Technology,Shenzhen,China
4.Department of Prosthodontics,School and Hospital of Stomatology,Wuhan University,Wuhan,China

Fan，L.,Ou，Y. J.,Zhu，Y. X.,et al. Lif Deficiency Leads to Iron Transportation Dysfunction in Ameloblasts[J]. JOURNAL OF DENTAL RESEARCH,2021.

Fan，L.,Ou，Y. J.,Zhu，Y. X.,Liang，Y. D.,Zhou，Y.,&Wang，Y. N..(2021).Lif Deficiency Leads to Iron Transportation Dysfunction in Ameloblasts.JOURNAL OF DENTAL RESEARCH.

Fan，L.,et al."Lif Deficiency Leads to Iron Transportation Dysfunction in Ameloblasts".JOURNAL OF DENTAL RESEARCH (2021).