Transcriptome-Wide Analysis of RNA m(6)A Methylation and Gene Expression Changes Among Two Arabidopsis Ecotypes and Their Reciprocal Hybrids

Xu, Zhihui1,2; Shi, Xiaobo2; Bao, Mengmei2; Song, Xiaoqian2; Zhang, Yuxia2; Wang, Haiyan2; Xie, Hairong2; Mao, Fei2; Wang, Shuai2; Jin, Hongmei3; Dong, Suomeng4; Zhang, Feng4; Wu, Zhe5; Wu, Yufeng2

2021-06-10

10.3389/fpls.2021.685189

Frontiers in Plant Science   影响因子和分区

1664-462X

The remodeling of transcriptome, epigenome, proteome, and metabolome in hybrids plays an important role in heterosis. N(6)-methyladenosine (m(6)A) methylation is the most abundant type of post-transcriptional modification for mRNAs, but the pattern of inheritance from parents to hybrids and potential impact on heterosis are largely unknown. We constructed transcriptome-wide mRNA m(6)A methylation maps of Arabidopsis thaliana Col-0 and Landsberg erecta (Ler) and their reciprocal F-1 hybrids. Generally, the transcriptome-wide pattern of m(6)A methylation tends to be conserved between accessions. Approximately 74% of m(6)A methylation peaks are consistent between the parents and hybrids, indicating that a majority of the m(6)A methylation is maintained after hybridization. We found a significant association between differential expression and differential m(6)A modification, and between non-additive expression and non-additive methylation on the same gene. The overall RNA m(6)A level between Col-0 and Ler is clearly different but tended to disappear at the allelic sites in the hybrids. Interestingly, many enriched biological functions of genes with differential m(6)A modification between parents and hybrids are also conserved, including many heterosis-related genes involved in biosynthetic processes of starch. Collectively, our study revealed the overall pattern of inheritance of mRNA m(6)A modifications from parents to hybrids and a potential new layer of regulatory mechanisms related to heterosis formation.

RNA m(6)A methylation hybrid heterosis Arabidopsis RNA modification dynamics

SCI

英语

其他

National Key Research and Development Program of China[2016YFD0101001] ; Fundamental Research Funds for the Central Universities[JCQY201901]

Plant Sciences

Plant Sciences

WOS:000665009100001

FRONTIERS MEDIA SA

Web of Science

1.Nanjing Agr Univ, Coll Life Sci, Nanjing, Peoples R China
2.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Acad Adv Interdisciplinary Studies, Bioinformat Ctr,Jiangsu Key Lab Informat Agr, Nanjing, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjing, Peoples R China
4.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Peoples R China
5.Southern Univ Sci & Technol, Dept Biol, SUSTech PKU Inst Plant & Food Sci, Shenzhen, Peoples R China

Xu, Zhihui,Shi, Xiaobo,Bao, Mengmei,et al. Transcriptome-Wide Analysis of RNA m(6)A Methylation and Gene Expression Changes Among Two Arabidopsis Ecotypes and Their Reciprocal Hybrids[J]. Frontiers in Plant Science,2021,12.

Xu, Zhihui.,Shi, Xiaobo.,Bao, Mengmei.,Song, Xiaoqian.,Zhang, Yuxia.,...&Wu, Yufeng.(2021).Transcriptome-Wide Analysis of RNA m(6)A Methylation and Gene Expression Changes Among Two Arabidopsis Ecotypes and Their Reciprocal Hybrids.Frontiers in Plant Science,12.

Xu, Zhihui,et al."Transcriptome-Wide Analysis of RNA m(6)A Methylation and Gene Expression Changes Among Two Arabidopsis Ecotypes and Their Reciprocal Hybrids".Frontiers in Plant Science 12(2021).