Biological sealing and integration of a fibrinogen-modified titanium alloy with soft and hard tissues in a rat model

Wang, Xiuli1; Lei, Xing2,3; Yu, Yue1; Miao, Sheng2; Tang, Jingyu1; Fu, Ye1; Ye, Kai1; Shen, Yang1; Shi, Jiayue1; Wu, Hao2; Zhu, Yi1; Yu, Lin1; Pei, Guoxian2,4; Bi, Long2; Ding, Jiandong1

2021-06-01

10.1039/d1bm00762a

Biomaterials Science   影响因子和分区

2047-4830

2047-4849

Percutaneous or transcutaneous devices are important and unique, and the corresponding biological sealing at the skin-implant interface is the key to their long-term success. Herein, we investigated the surface modification to enhance biological sealing, using a metal sheet and screw bonded by biomacromolecule fibrinogen mediated via pre-deposited synthetic macromolecule polydopamine (PDA) as a demonstration. We examined the effects of a Ti-6Al-4V titanium alloy modified with fibrinogen (Ti-Fg), PDA (Ti-PDA) or their combination (Ti-PDA-Fg) on the biological sealing and integration with skin and bone tissues. Human epidermal keratinocytes (HaCaT), human foreskin fibroblasts (HFF) and preosteoblasts (MC3T3-E1), which are closely related to percutaneous implants, exhibited better adhesion and spreading on all the three modified sheets compared with the unmodified alloy. After three-week subcutaneous implantation in Sprague-Dawley (SD) rats, the Ti-PDA-Fg sheets could significantly attenuate the soft tissue response and promote angiogenesis compared with other groups. Furthermore, in the model of percutaneous tibial implantation in SD rats, the Ti-PDA-Fg screws dramatically inhibited epithelial downgrowth and promoted new bone formation. Hence, the covalent immobilization of fibrinogen through the precoating of PDA is promising for enhanced biological sealing and osseointegration of metal implants with soft and hard tissues, which is critical for an orthopedic percutaneous medical device.

SCI ; EI

英语

其他

National Key R&D Program of China[2016YFC1100300] ; National Science Foundation of China[21961160721,81672189] ; Linyi Municipal Science and Technology Development Plan[201818015]

Materials Science

Materials Science, Biomaterials

WOS:000664578900001

ROYAL SOC CHEMISTRY

20213110704837

Aluminum alloys ; Cell culture ; Histology ; Implants (surgical) ; Rats ; Screws ; Sheet metal ; Ternary alloys ; Tissue ; Tissue regeneration ; Vanadium alloys

Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Prosthetics:462.4 ; Aluminum Alloys:541.2 ; Titanium and Alloys:542.3 ; Vanadium and Alloys:543.6 ; Small Tools and Hardware:605

Web of Science

1.Fudan Univ, Dept Macromol Sci, State Key Lab Mol Engn Polymers, Shanghai 200438, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp, Dept Orthoped, Xian 710032, Peoples R China
3.Linyi Peoples Hosp, Dept Orthoped Surg, Linyi 276000, Shandong, Peoples R China
4.Southern Univ Sci & Technol, Southern Univ Sci & Technol Hosp, Shenzhen 518055, Peoples R China

Wang, Xiuli,Lei, Xing,Yu, Yue,et al. Biological sealing and integration of a fibrinogen-modified titanium alloy with soft and hard tissues in a rat model[J]. Biomaterials Science,2021,9:5192-5208.

Wang, Xiuli.,Lei, Xing.,Yu, Yue.,Miao, Sheng.,Tang, Jingyu.,...&Ding, Jiandong.(2021).Biological sealing and integration of a fibrinogen-modified titanium alloy with soft and hard tissues in a rat model.Biomaterials Science,9,5192-5208.

Wang, Xiuli,et al."Biological sealing and integration of a fibrinogen-modified titanium alloy with soft and hard tissues in a rat model".Biomaterials Science 9(2021):5192-5208.