Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells

Luo, Hai-Tao1,2,3,4; Zheng, Yuan-Yuan1,2,3; Tang, Jun2,4,5; Shao, Li-Juan1,2,3,4; Mao, Yi-Heng6; Yang, Wei1,2,3; Yang, Xiao-Fei1,2,3; Li, Yang1,2,3; Tian, Rui-Jun6; Li, Fu-Rong1,2,3

2021-06-14

10.1038/s41525-021-00217-5

NPJ GENOMIC MEDICINE   影响因子和分区

2056-7944

Lung adenocarcinoma is heterogeneous and hierarchically organized, with a subpopulation of stem-like cells (CSCs) that reside at the apex of the hierarchy, in which exosomes act as important mediators by transporting specific molecules among different cell populations. Although there have been numerous studies on tumor exosomes, the constituents and functional properties of CSC-derived exosomes are still poorly characterized. Here we present a detail transcriptome and proteome atlas of the exosomes released by human lung adenocarcinoma stem-like cells (LSLCs). The transcriptome analysis indicates the specific patterns of exosomal constituents, including the fragmentation of transcripts and the low-level presence of circular RNAs, and identifies multiple exosomal-enriched mRNAs and lncRNAs. Integrative analysis of transcriptome and proteome data reveals the diverse functions of exosomal-enriched RNAs and proteins, many of which are associated with tumorigenesis. Importantly, several LSLC markers we identified are highly expressed in LSLC-derived exosomes and associate with poor survival, which may serve as promising liquid biopsy biomarkers for lung adenocarcinoma diagnosis. Our study provides a resource for the future elucidation of the functions of tumor-derived exosomes and their regulatory mechanisms in mediating lung cancer development.

SCI

英语

通讯

Science and Technology Project of Shenzhen["ZDSYS20190902093203727","JCYJ20170413092711058","GJHZ20170310090257380","JCYJ20190807145013281"] ; National Natural Science Foundation of China[81602695] ; China Postdoctoral Science Foundation[2019M663369]

Genetics & Heredity

Genetics & Heredity

WOS:000664119500002

NATURE RESEARCH

2-s2.0-85107867968

Web of Science

1.Jinan Univ, Translat Med Collaborat Innovat Ctr, Shenzhen Peoples Hosp, Second Clin Med Coll, Shenzhen, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen, Guangdong, Peoples R China
3.Shenzhen Key Lab Stem Cell Res & Clin Transformat, Shenzhen, Peoples R China
4.Jinan Univ, Integrated Chinese & Western Med Postdoctoral Res, Guangzhou, Peoples R China
5.Jinan Univ, Inst Oncol, Shenzhen Peoples Hosp, Second Clin Med Coll, Shenzhen, Guangdong, Peoples R China
6.Southern Univ Sci & Technol, Dept Chem, Shenzhen, Peoples R China

Luo, Hai-Tao,Zheng, Yuan-Yuan,Tang, Jun,et al. Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells[J]. NPJ GENOMIC MEDICINE,2021,6(1).

Luo, Hai-Tao.,Zheng, Yuan-Yuan.,Tang, Jun.,Shao, Li-Juan.,Mao, Yi-Heng.,...&Li, Fu-Rong.(2021).Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells.NPJ GENOMIC MEDICINE,6(1).

Luo, Hai-Tao,et al."Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells".NPJ GENOMIC MEDICINE 6.1(2021).