Cytokine/Chemokine Expression Is Closely Associated Disease Severity of Human Adenovirus Infections in Immunocompetent Adults and Predicts Disease Progression

Li, Jin1; Wei, Jinli2; Xu, Zhixiang2; Jiang, Chunmei3; Li, Mianhuan2; Chen, Jie4; Li, Yanjie2; Yang, Minghui1; Gu, Yuchen2; Wang, Fuxiang2; Shu, Yuelong1; Yang, Yang2; Sun, Litao1; Liu, Yingxia2

2021-06-07

10.3389/fimmu.2021.691879

Frontiers in Immunology   影响因子和分区

1664-3224

Increasing human Adenovirus (HAdV) infections complicated with acute respiratory distress syndrome (ARDS) even fatal outcome were reported in immunocompetent adolescent and adult patients. Here, we characterized the cytokine/chemokine expression profiles of immunocompetent patients complicated with ARDS during HAdV infection and identified biomarkers for disease severity/progression. Forty-eight cytokines/chemokines in the plasma samples from 19 HAdV-infected immunocompetent adolescent and adult patients (ten complicated with ARDS) were measured and analyzed in combination with clinical indices. Immunocompetent patients with ARDS caused by severe acute respiratory disease coronavirus (SARS-CoV)-2, 2009 pandemic H1N1 (panH1N1) or bacteria were included for comparative analyses. Similar indices of disease course/progression were found in immunocompetent patients with ARDS caused by HAdV, SARS-CoV-2 or panH1N infections, whereas the HAdV-infected group showed a higher prevalence of viremia, as well as increased levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatine kinase (CK). Expression levels of 33 cytokines/chemokines were increased significantly in HAdV-infected patients with ARDS compared with that in healthy controls, and many of them were also significantly higher than those in SARS-CoV-2-infected and panH1N1-infected patients. Expression of interferon (IFN)-gamma, interleukin (IL)-1 beta, hepatocyte growth factor (HGF), monokine induced by IFN-gamma (MIG), IL-6, macrophage-colony stimulating factor (M-CSF), IL-10, IL-1 alpha and IL-2Ra was significantly higher in HAdV-infected patients with ARDS than that in those without ARDS, and negatively associated with the ratio of the partial pressure of oxygen in arterial blood/fraction of inspired oxygen (PaO2/FiO(2)). Analyses of the receiver operating characteristic curve (ROC) showed that expression of IL-10, M-CSF, MIG, HGF, IL-1 beta, IFN-gamma and IL-2Ra could predict the progression of HAdV infection, with the highest area under the curve (AUC) of 0.944 obtained for IL-10. Of note, the AUC value for the combination of IL-10, IFN-gamma, and M-CSF reached 1. In conclusion, the "cytokine storm" occurred during HAdV infection in immunocompetent patients, and expression of IL-10, M-CSF, MIG, HGF, IL-1 beta, IFN-gamma and IL-2Ra was closely associated with disease severity and could predict disease progression.

human adenoviruses cytokine storm prediction immunocompetent adults disease progression

SCI

英语

通讯

Shenzhen Science and Technology Research and Development Project[202002073000001,"JSGG20200225152008136"] ; National Natural Science Foundation of China[31971147] ; Shenzhen Science and Technology Innovation Commission["JCYJ20190807155011406","KQTD20180411143323605"] ; National Science and Technology Major Project[2018ZX10713001] ; Ministry of Science and Technology[2020YFC0846300]

Immunology

Immunology

WOS:000663752500001

FRONTIERS MEDIA SA

Web of Science

1.Sun Yat Sen Univ, Sch Publ Hlth Shenzhen, Shenzhen, Peoples R China
2.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Hosp 2,Natl Clin Res Ctr Infect Dis, State Key Discipline Infect Dis,Shenzhen Key Lab, Shenzhen, Peoples R China
3.Peoples Hosp Longhua, Dept Infect Dis, Shenzhen, Peoples R China
4.Guangzhou Sagene Biotech Co Ltd, Res & Dev Dept, Guangzhou, Peoples R China

Li, Jin,Wei, Jinli,Xu, Zhixiang,et al. Cytokine/Chemokine Expression Is Closely Associated Disease Severity of Human Adenovirus Infections in Immunocompetent Adults and Predicts Disease Progression[J]. Frontiers in Immunology,2021,12.

Li, Jin.,Wei, Jinli.,Xu, Zhixiang.,Jiang, Chunmei.,Li, Mianhuan.,...&Liu, Yingxia.(2021).Cytokine/Chemokine Expression Is Closely Associated Disease Severity of Human Adenovirus Infections in Immunocompetent Adults and Predicts Disease Progression.Frontiers in Immunology,12.

Li, Jin,et al."Cytokine/Chemokine Expression Is Closely Associated Disease Severity of Human Adenovirus Infections in Immunocompetent Adults and Predicts Disease Progression".Frontiers in Immunology 12(2021).