南方科技大学知识苑(SUSTech KC): LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3

题名	LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3
作者	Liu, Yingnan 1,2; Zhuang, Yongqing 1,2; Fu, Xiaokuan 1,2; Li, Chaofei 3
通讯作者	Li, Chaofei
发表日期	2021-07-09
DOI	10.1007/s12672-021-00414-9
发表期刊	Hormones & Cancer 影响因子和分区
ISSN	1868-8497
EISSN	2730-6011
卷号	12 期号:1
摘要	Background LncRNA POU3F3 (POU3F3) is overexpressed and plays oncogenic roles in esophageal squamous-cell carcinomas. LncRNA MEG3 (MEG3) has been characterized as a tumor suppressive lncRNA in different types of cancer. Our preliminary deep sequencing analysis revealed the inverse correlation between POU3F3 and MEG2 across melanoma tissues, indicating the interaction between them in melanoma. Therefore, this study was performed to investigate the crosstalk between POU3F3 and MEG3 in melanoma. Methods Tumor and adjacent healthy tissues collected from 60 melanoma patients were subjected to RNA extractions and RT-qPCRs to analyze the differential expression of POU3F3 and MEG2 in melanoma. In melanoma cells, POU3F3 and MEG2 were overexpressed to study the interactions between them. CCK-8 assays were performed to analyze the roles of POU3F3 and MEG2 in regulating melanoma cell proliferation. Results We found that POU3F3 was upregulated, while lncRNA MEG3 was downregulated in melanoma. Expression levels of POU3F3 and MEG3 were inversely correlated across tumor tissues. In vitro experiments showed that POU3F3 overexpression decreased MEG3 expression in melanoma cells, while MEG3 overexpression failed to affect POU3F3. POU3F3 overexpression increased melanoma cell proliferation, while MEG3 overexpression decreased melanoma cell proliferation. In addition, rescue experiments showed that MEG3 overexpression attenuated the enhancing effects of POU3F3 overexpression. Conclusion POU3F3 may promote melanoma cell proliferation by downregulating MEG3.
关键词	Melanoma LncRNA MEG3 Proliferation
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	Sanming Project of Medicine in Shenzhen[SZSM201512032]
WOS研究方向	Oncology ; Endocrinology & Metabolism
WOS类目	Oncology ; Endocrinology & Metabolism
WOS记录号	WOS:000671571800001
出版者	SPRINGER
来源库	Web of Science
引用统计	被引频次[WOS]：8
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/240186
专题	南方科技大学第一附属医院
作者单位	1.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Hand & Microvasc Surg, Shenzhen 518000, Guangdong, Peoples R China 2.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Dept Hand & Microvasc Surg, Shenzhen 518000, Guangdong, Peoples R China 3.Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Dept Gen Surg, 12th Floor,Bldg 9,197,Ruijin 2nd Rd, Shanghai 200025, Peoples R China
第一作者单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Liu, Yingnan,Zhuang, Yongqing,Fu, Xiaokuan,et al. LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3[J]. Hormones & Cancer,2021,12(1).
APA	Liu, Yingnan,Zhuang, Yongqing,Fu, Xiaokuan,&Li, Chaofei.(2021).LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3.Hormones & Cancer,12(1).
MLA	Liu, Yingnan,et al."LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3".Hormones & Cancer 12.1(2021).