A Gelatin-Hyaluronic Acid Double Cross-Linked Hydrogel for Regulating the Growth and Dual Dimensional Cartilage Differentiation of Bone Marrow Mesenchymal Stem Cells

Ren, Ying1; Zhang, Yuan3; Zhang, Han1; Wang, Yunping1; Liu, Lingrong1; Zhang, Qiqing1,2,3

2021-06-01

10.1166/jbn.2021.3088

Journal of Biomedical Nanotechnology   影响因子和分区

1550-7033

1550-7041

Owing to its unique physiochemical properties similar to the extracellular matrix (ECM), three-dimensional (3D) cross linked hydrogels are widely studied materials for tissue engineering. In this study, to mimic the ECM microenvironment, a two-step covalent cross-linking with hyaluronic acid and gelatin was performed to form an interpenetrating polymer network structure. Gelatin as the first network greatly improved the mechanical strength of the hydrogels, while a hyaluronic acid IP: 12700 1 On Wed 30 Jun 2021 17 50:47 network as the second network improved the tenacity and biological ativity. Compared with a single network hydrogel, Copyright: American Scient fic Pubishers the interpenetrating hydrogel system can further regulatDeliveredthe bymechaniclIngenta properties of the hydrogel by adjusting the ratio of the two components, thereby changing the proliferation, activity, and direction of cartilage differentiation of bone marrow mesenchymal stem cells (BMSCs). Not only that, with two culture methods for BMSCs on the surface and 3D wrapped in the double cross-linked hydrogels, they exhibited their potential to induce BMSCs to cartilage differentiation under the condition of 3D encapsulation of BMSCs and contact with BMSCs on its surface. As a scaffold material for cartilage tissue engineering, this double cross-linked hydrogel demonstrated its high feasibility and applicability in delivering BMSCs in vivo and repairing defects.

Gelatin Hyaluronic Acid Double Cross-Linked Hydrogel Bone Marrow Mesenchymal Stem Cell ECM Microenvironment Cartilage Differentiation

SCI

英语

通讯

National Key Basic Research and Development Projects["2017YFC1103601","2017YFC1104102"] ; Key Project of GuangDong Basic and Applied Basic Research Foundation[2020B1515120091]

Science & Technology - Other Topics ; Materials Science

Nanoscience & Nanotechnology ; Materials Science, Biomaterials

WOS:000668347100002

AMER SCIENTIFIC PUBLISHERS

Web of Science

1.Chinese Acad Med Sci & Peking Union Med Coll, Inst Biomed Engn, Tianjin Key Lab Biomed Mat, Tianjin 300192, Peoples R China
2.South Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Inst Biomed Engn, Shenzhen 518020, Guangdong, Peoples R China
3.Fujian Bote Biotechnol Co Ltd, Fuzhou 350013, Fujian, Peoples R China

Ren, Ying,Zhang, Yuan,Zhang, Han,et al. A Gelatin-Hyaluronic Acid Double Cross-Linked Hydrogel for Regulating the Growth and Dual Dimensional Cartilage Differentiation of Bone Marrow Mesenchymal Stem Cells[J]. Journal of Biomedical Nanotechnology,2021,17(6).

Ren, Ying,Zhang, Yuan,Zhang, Han,Wang, Yunping,Liu, Lingrong,&Zhang, Qiqing.(2021).A Gelatin-Hyaluronic Acid Double Cross-Linked Hydrogel for Regulating the Growth and Dual Dimensional Cartilage Differentiation of Bone Marrow Mesenchymal Stem Cells.Journal of Biomedical Nanotechnology,17(6).

Ren, Ying,et al."A Gelatin-Hyaluronic Acid Double Cross-Linked Hydrogel for Regulating the Growth and Dual Dimensional Cartilage Differentiation of Bone Marrow Mesenchymal Stem Cells".Journal of Biomedical Nanotechnology 17.6(2021).