Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregation

Xu, Leilei1,2; Ali, Mahboob1; Duan, Wenxiu3; Yuan, Xiao1,4; Garba, Fatima2; Mullen, McKay1,2; Sun, Binwen5; Poser, Ina6; Duan, Hequan1,2,3; Lu, Jianlin1; Tian, Ruijun4; Ge, Yushu3; Chu, Lingluo1,2; Pan, Weijun7; Wang, Dongmei1; Hyman, Anthony6; Green, Hadiyah2; Li, Lin7; Dou, Zhen1,3; Liu, Dan3; Liu, Xing1,2; Yao, Xuebiao1,2

2021-07-13

10.1016/j.celrep.2021.109343

Cell Reports   影响因子和分区

2211-1247

Stable transmission of genetic material during cell division requires accurate chromosome segregation. PLK1 dynamics at kinetochores control establishment of correct kinetochore-microtubule attachments and subsequent silencing of the spindle checkpoint. However, the regulatory mechanism responsible for PLK1 activity in prometaphase has not yet been affirmatively identified. Here we identify Apolo1, which tunes PLK1 activity for accurate kinetochore-microtubule attachments. Apolo1 localizes to kinetochores during early mitosis, and suppression of Apolo1 results in misaligned chromosomes. Using the fluorescence resonance energy transfer (FRET)-based PLK1 activity reporter, we found that Apolo1 sustains PLK1 kinase activity at kinetochores for accurate attachment during prometaphase. Apolo1 is a cognate substrate of PLK1, and the phosphorylation enables PP1 gamma to inactivate PLK1 by dephosphorylation. Mechanistically, Apolo1 constitutes a bridge between kinase and phosphatase, which governs PLK1 activity in prometaphase. These findings define a previously uncharacterized feedback loop by which Apolo1 provides fine-tuning for PLK1 to guide chromosome segregation in mitosis.

SCI

英语

通讯

National Key Research and Development Program of China["2017YFA0503600","2016YFA0100500","2016YFA0101200"] ; Chinese Natural Science Foundation[31621002,32090040,21922706,91854203,91853115,81630080,31871359,21778050,91749125,31430054,31970655,31471275] ; Ministry of Education["IRT_17R102",20113402130010] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB19000000] ; Fundamental Research Funds for the Central Universities[WK2070000194] ; Anhui Province Key Project[08040102005] ; Chinese Academy of Sciences Center of Excellence[2015HSC-UE010] ; Anhui Provincial Natural Science Foundation[1508085SMC213] ; independent Innovation Foundation of theUniversity of Science and Technology of China["WK2070000111","YD2070006001","WK2070000123"] ; National Institutes of Health (United States)["CA164133","DK115812","DK56292"] ; European Commission's Seventh Framework Program[FP7-HEALTH-2009-241548]

Cell Biology

Cell Biology

WOS:000672741900005

CELL PRESS

Web of Science

1.Univ Sci & Technol China, MOE Key Lab Membraneless Organelles & Cellular Dy, Hefei 230027, Peoples R China
2.Morehouse Sch Med, Keck Ctr Mol Imaging, Atlanta, GA 30310 USA
3.Hefei Natl Ctr Phys Sci Microscale, Anhui Key Lab Chem Biol, Hefei 230027, Peoples R China
4.Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Peoples R China
5.Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
6.Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany
7.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China

Xu, Leilei,Ali, Mahboob,Duan, Wenxiu,et al. Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregation[J]. Cell Reports,2021,36(2).

Xu, Leilei.,Ali, Mahboob.,Duan, Wenxiu.,Yuan, Xiao.,Garba, Fatima.,...&Yao, Xuebiao.(2021).Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregation.Cell Reports,36(2).

Xu, Leilei,et al."Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregation".Cell Reports 36.2(2021).