Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063

Xiaol, Yanan1,2; Huang, Guoqiang3,4; You, Xin3,4; Zhu, Qingjun1,2; Wang, Wenda1; Kuang, Tingyun1; Han, Guangye1; Sui, Sen-Fang3,4,5; Shen, Jian-Ren1,6,7

2021-07-01

10.1038/s41477-021-00961-7

Nature Plants   影响因子和分区

2055-026X

2055-0278

["Cryo-electron microscopy structures of two PSII intermediates from psbV-deletion and wild-type strains of cyanobacteria show substantial structural changes. These provide a structural basis for understanding the assembly process of native PSII.","Photosystem II (PSII) is a multisubunit pigment-protein complex and catalyses light-induced water oxidation, leading to the conversion of light energy into chemical energy and the release of dioxygen. We analysed the structures of two Psb28-bound PSII intermediates, Psb28-RC47 and Psb28-PSII, purified from a psbV-deletion strain of the thermophilic cyanobacterium Thermosynechococcus vulcanus, using cryo-electron microscopy. Both Psb28-RC47 and Psb28-PSII bind one Psb28, one Tsl0063 and an unknown subunit. Psb28 is located at the cytoplasmic surface of PSII and interacts with D1, D2 and CP47, whereas Tsl0063 is a transmembrane subunit and binds at the side of CP47/PsbH. Substantial structural perturbations are observed at the acceptor side, which result in conformational changes of the quinone (Q(B)) and non-haem iron binding sites and thus may protect PSII from photodamage during assembly. These results provide a solid structural basis for understanding the assembly process of native PSII."]

SCI

英语

通讯

National Key R&D Program of China["2017YFA0503700","2016YFA0501101","2017YFA0504600","2020YFA0907600","2019YFA0906300"] ; National Natural Science Foundation of China[31470339] ; Strategic Priority Research Program of CAS["XDA27050402","XDB17000000"] ; CAS Key Research programme for Frontier Science[QYZDY-SSW-SMC003] ; Youth Innovation Promotion Association of CAS[2020081] ; CAS Interdisciplinary Innovation Team[JCTD-2020-06]

Plant Sciences

Plant Sciences

WOS:000669838400005

NATURE RESEARCH

Web of Science

1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Tsinghua Univ, Sch Life Sci, State Key Lab Membrane Biol, Beijing Adv Innovat Ctr Struct Biol, Beijing, Peoples R China
4.Tsinghua Univ, Frontier Res Ctr Biol Struct, Sch Life Sci, Beijing, Peoples R China
5.Southern Univ Sci & Technol, Dept Biol, Shenzhen, Peoples R China
6.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama, Japan
7.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama, Japan

Xiaol, Yanan,Huang, Guoqiang,You, Xin,et al. Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063[J]. Nature Plants,2021.

Xiaol, Yanan.,Huang, Guoqiang.,You, Xin.,Zhu, Qingjun.,Wang, Wenda.,...&Shen, Jian-Ren.(2021).Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063.Nature Plants.

Xiaol, Yanan,et al."Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063".Nature Plants (2021).