Monitoring Metabolites Using an NAD(P)H-sensitive Polymer Dot and a Metabolite-Specific Enzyme

Chen, Haobin1; Yu, Jiangbo1; Zhang, Jicheng1; Sun, Kai1; Ding, Zhaoyang1; Jiang, Yifei1; Hu, Qiongzheng1; Wu, Changfeng2; Chiu, Daniel T.1

2021-07-01

10.1002/anie.202106156

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   影响因子和分区

1433-7851

1521-3773

We introduce an NAD(P)H-sensitive polymer dot (Pdot) biosensor for point-of-care monitoring of metabolites. The Pdot is combined with a metabolite-specific NAD(P)H-dependent enzyme that catalyzes the oxidation of the metabolite, generating NAD(P)H. Upon UV illumination, the NAD(P)H quenches the fluorescence emission of Pdot at 627 nm via electron transfer, and also fluoresces at 458 nm, resulting in a shift from red to blue emission at higher NAD(P)H concentrations. Metabolite concentration is quantified ratiometrically-based on the ratio of blue-to-red channel emission intensities, with a digital camera-with high sensitivity and specificity. We demonstrate phenylalanine biosensing in human plasma for a phenylketonuria screening test, quantifying several other disease-related metabolites (lactate, glucose, glutamate, and beta-hydroxybutyrate), and a paper-based assay with smartphore imaging for point-of-care use.

metabolite biosensors nicotinamide adenine dinucleotide paper assays point of care semiconducting polymer dots

SCI ; EI

英语

NI论文

其他

Chemistry

Chemistry, Multidisciplinary

WOS:000677814100001

WILEY-V C H VERLAG GMBH

20213010674502

Amino acids ; Biomolecules ; Diagnosis ; Electron transport properties ; Enzymes

Medicine and Pharmacology:461.6 ; Biology:461.9 ; Organic Compounds:804.1

CHEMISTRY

Web of Science

1.Univ Washington, Dept Chem & Bioengn, Seattle, WA 98195 USA
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 510855, Guangdong, Peoples R China

Chen, Haobin,Yu, Jiangbo,Zhang, Jicheng,et al. Monitoring Metabolites Using an NAD(P)H-sensitive Polymer Dot and a Metabolite-Specific Enzyme[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2021,60(35):19331-19336.

Chen, Haobin.,Yu, Jiangbo.,Zhang, Jicheng.,Sun, Kai.,Ding, Zhaoyang.,...&Chiu, Daniel T..(2021).Monitoring Metabolites Using an NAD(P)H-sensitive Polymer Dot and a Metabolite-Specific Enzyme.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,60(35),19331-19336.

Chen, Haobin,et al."Monitoring Metabolites Using an NAD(P)H-sensitive Polymer Dot and a Metabolite-Specific Enzyme".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 60.35(2021):19331-19336.