Instrumentation-Compact Digital Microfluidic Reaction Interface-Extended Loop-Mediated Isothermal Amplification for Sample-to-Answer Testing of Vibrio parahaemolyticus

Zheng, Guoxia1,2; Gao, Qian1; Jiang, Youwei3; Lu, Ling1; Li, Jianfeng4; Zhang, Xingcai5; Zhao, Hongyu2; Fan, Panpan1; Cui, Yutong2; Gu, Furong2; Wang, Yunhua1

2021-07-20

10.1021/acs.analchem.1c00917

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Vibrio parahaemolyticus is usually spread via consumption of contaminated seafood and causes vibriosis. By combination of digital microfluidic (DMF) and loop-mediated isothermal amplification (LAMP), we provided an automated instrumentation-compact DMF- LAMP device for sample-to-answer detection of V. parahaemolyticus. For the first time, how much the proper mixing might facilitate the DMF-LAMP process is explored. The results illustrated that increasing the number of flow configurations and decreasing the fluid-reversibility will extend the interfacial surface available for diffusion-based mass transfer within a droplet microreactor, thus contributing to the overall amplification reaction rate. Noticeably, the DMF-LAMP amplification plateau time is shortened by proper mixing, from 60 min in static mixing and traditional bulk LAMP to 30 min in 2-electrode mixing and 15 min in 3-electrode mixing. The device achieved much higher detection sensitivity (two copies per reaction) than previously reported devices. V. parahaemolyticus from spiked shrimps is detected by Q-tip sampling associated with 3-electrode mixing DMF-LAMPs. The detectable signal occurs within only 3 min at a higher concentration and, at most, is delayed to 18 min, with a detection limit of <0.23 x 10(3) CFU/g. Thus, the developed DMF-LAMP device demonstrates potential for being used as a sample-to-answer system with a quick analysis time, high sensitivity, and sample-to-answer format.

SCI ; EI

英语

NI论文

其他

National Natural Science Foundation of China[41476085,81471807] ; Dalian Science and Technology Bureau (Dalian Science and Technology Innovation Fund)[2019J12SN55] ; Major Scientific Project of Interscholastic Collaboration of Universities of Liaoning[JYT-dldxjc-202001]

Chemistry

Chemistry, Analytical

WOS:000677481700010

AMER CHEMICAL SOC

20213010676484

Digital microfluidics ; Isotherms ; Mass transfer ; Mixing

Microfluidics:632.5.1 ; Mass Transfer:641.3 ; Chemical Operations:802.3

CHEMISTRY

Web of Science

1.Dalian Univ, Med Sch, Dalian 116622, Peoples R China
2.Dalian Univ, Chem & Environm Engn Inst, Dalian 116622, Peoples R China
3.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Peoples R China
4.Jiangsu Celyee Cell Technol Res Inst Co, Nanjing 210000, Peoples R China
5.Harvard Univ, Sch Engn & Appl Sci, Dept Phys, Cambridge, MA 02138 USA

Zheng, Guoxia,Gao, Qian,Jiang, Youwei,et al. Instrumentation-Compact Digital Microfluidic Reaction Interface-Extended Loop-Mediated Isothermal Amplification for Sample-to-Answer Testing of Vibrio parahaemolyticus[J]. ANALYTICAL CHEMISTRY,2021,93(28):9728-9736.

Zheng, Guoxia.,Gao, Qian.,Jiang, Youwei.,Lu, Ling.,Li, Jianfeng.,...&Wang, Yunhua.(2021).Instrumentation-Compact Digital Microfluidic Reaction Interface-Extended Loop-Mediated Isothermal Amplification for Sample-to-Answer Testing of Vibrio parahaemolyticus.ANALYTICAL CHEMISTRY,93(28),9728-9736.

Zheng, Guoxia,et al."Instrumentation-Compact Digital Microfluidic Reaction Interface-Extended Loop-Mediated Isothermal Amplification for Sample-to-Answer Testing of Vibrio parahaemolyticus".ANALYTICAL CHEMISTRY 93.28(2021):9728-9736.