rpoS-mutation variants are selected in Pseudomonas aeruginosa biofilms under imipenem pressure

Duan, Xiangke1,2; Pan, Yanrong2; Cai, Zhao2; Liu, Yumei2; Zhang, Yingdan2; Liu, Moxiao2; Liu, Yang3; Wang, Ke4; Zhang, Lianhui1; Yang, Liang2,5

2021-07-21

10.1186/s13578-021-00655-9

CELL AND BIOSCIENCE   影响因子和分区

2045-3701

Background Pseudomonas aeruginosa is a notorious opportunistic pathogen causing various types of biofilm-related infections. Biofilm formation is a unique microbial strategy that allows P. aeruginosa to survive adverse conditions such as antibiotic treatment and human immune clearance. Results In this study, we experimentally evolved P. aeruginosa PAO1 biofilms for cyclic treatment in the presence of high dose of imipenem, and enriched hyperbiofilm mutants within six cycles in two independent lineages. The competition assay showed that the evolved hyperbiofilm mutants can outcompete the ancestral strain within biofilms but not in planktonic cultures. Whole-genome sequencing analysis revealed the hyperbiofilm phenotype is caused by point mutations in rpoS gene in all independently evolved mutants and the same mutation was found in P. aeruginosa clinical isolates. We further showed that mutation in rpoS gene increased the intracellular c-di-GMP level by turning on the expression of the diguanylate cyclases. Mutation in rpoS increased pyocyanin production and virulence in hyperbiofilm variants. Conclusion Here, our study revealed that antibiotic treatment of biofilm-related P. aeruginosa infections might induce a hyperbiofilm phenotype via rpoS mutation, which might partially explain antimicrobial treatment failure of many P. aeruginosa biofilm-related infections.

Experimental biofilm evolution Pseudomonas aeruginosa Sigma factor RpoS Biofilms Cyclic-di-GMP Virulence

SCI

英语

通讯

Shenzhen Key Laboratory of Gene Regulation and Systems Biology[ZDSYS20200811144002008] ; Shenzhen Overseas High-level Talent Team[KQTD20200909113758004] ; Guangdong Natural Science Foundation for Distinguished Young Scholar[2020B1515020003] ; Guangdong Natural Science Foundation["2019A1515110640","2020A1515010316"] ; National Natural Science Foundation of China[81760024] ; Guangzhou Municipal Science and Technology Bureau[201607020044]

Biochemistry & Molecular Biology

Biochemistry & Molecular Biology

WOS:000677596800001

BMC

Web of Science

1.South China Agr Univ, Integrat Microbiol Res Ctr, Guangdong Prov Key Lab Microbial Signals & Dis Co, Guangzhou 510642, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Sch Med, Guangzhou 518055, Guangdong, Peoples R China
3.Southern Univ Sci & Technol Hosp, Shenzhen 518055, Guangdong, Peoples R China
4.Guangxi Med Univ, Affiliated Hosp 1, Dept Pulm & Crit Care Med, Nanning 530021, Guangxi, Peoples R China
5.Southern Univ Sci & Technol, Shenzhen Key Lab Gene Regulat & Syst Biol, Shenzhen 518055, Guangdong, Peoples R China

Duan, Xiangke,Pan, Yanrong,Cai, Zhao,et al. rpoS-mutation variants are selected in Pseudomonas aeruginosa biofilms under imipenem pressure[J]. CELL AND BIOSCIENCE,2021,11(1).

Duan, Xiangke.,Pan, Yanrong.,Cai, Zhao.,Liu, Yumei.,Zhang, Yingdan.,...&Yang, Liang.(2021).rpoS-mutation variants are selected in Pseudomonas aeruginosa biofilms under imipenem pressure.CELL AND BIOSCIENCE,11(1).

Duan, Xiangke,et al."rpoS-mutation variants are selected in Pseudomonas aeruginosa biofilms under imipenem pressure".CELL AND BIOSCIENCE 11.1(2021).