DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations

Khalil，Ibrahim1,2; Hashem，Abu1,3; Nath，Amit R.1,4; Muhd Julkapli，Nurhidayatullaili1; Yehye，Wageeh A.1; Basirun，Wan Jeffrey1,5

2021-10-01

10.1016/j.mcp.2021.101758

MOLECULAR AND CELLULAR PROBES   影响因子和分区

0890-8508

1096-1194

Authentication, detection and quantification of ingredients, and adulterants in food, meat, and meat products are of high importance these days. The conventional techniques for the detection of meat species based on lipid, protein and DNA biomarkers are facing challenges due to the poor selectivity, sensitivity and unsuitability for processed food products or complex food matrices. On the other hand, DNA based molecular techniques and nanoparticle based DNA biosensing strategies are gathering huge attention from the scientific communities, researchers and are considered as one of the best alternatives to the conventional strategies. Though nucleic acid based molecular techniques such as PCR and DNA sequencing are getting greater successes in species detection, they are still facing problems from its point-of-care applications. In this context, nanoparticle based DNA biosensors have gathered successes in some extent but not to a satisfactory stage to mark with. In recent years, many articles have been published in the area of progressive nucleic acid-based technologies, however there are very few review articles on DNA nanobiosensors in food science and technology. In this review, we present the fundamentals of DNA based molecular techniques such as PCR, DNA sequencing and their applications in food science. Moreover, the in-depth discussions of different DNA biosensing strategies or more specifically electrochemical and optical DNA nanobiosensors are presented. In addition, the significance of DNA nanobiosensors over other advanced detection technologies is discussed, focusing on the deficiencies, advantages as well as current challenges to ameliorate with the direction for future development.

Biosensors DNA Electrochemical Nanomaterials Optical PCR

SCI

英语

其他

University Malaya Research Grant[UMRG RP044A-17AET]

Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Cell Biology

Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Cell Biology

WOS:000702877700005

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD

MOLECULAR BIOLOGY & GENETICS

2-s2.0-85110572714

Scopus

1.Nanotechnology and Catalysis Research Center (NANOCAT),Institute for Advanced Studies (IAS),Universiti Malaya,Kuala Lumpur,50603,Malaysia
2.Healthcare Pharmaceuticals Ltd.,Gazipur,Rajendrapur,Bangladesh
3.Microbial Biotechnology Division,National Institute of Biotechnology,Ashulia,Ganakbari, Savar,1349,Bangladesh
4.Shenzhen Grubbs Institute and Department of Chemistry,Southern University of Science and Technology,518055,China
5.Department of Chemistry,Universiti Malaya,Malaysia

Khalil，Ibrahim,Hashem，Abu,Nath，Amit R.,et al. DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations[J]. MOLECULAR AND CELLULAR PROBES,2021,59.

Khalil，Ibrahim,Hashem，Abu,Nath，Amit R.,Muhd Julkapli，Nurhidayatullaili,Yehye，Wageeh A.,&Basirun，Wan Jeffrey.(2021).DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations.MOLECULAR AND CELLULAR PROBES,59.

Khalil，Ibrahim,et al."DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations".MOLECULAR AND CELLULAR PROBES 59(2021).