An easy-to-operate method for single-cell isolation and retrieval using a microfluidic static droplet array

Ding，Lin1; Radfar，Payar1; Rezaei，Meysam1,2,3; Warkiani，Majid Ebrahimi1,2,3,4

2021-08-01

10.1007/s00604-021-04897-9

MICROCHIMICA ACTA   影响因子和分区

0026-3672

1436-5073

In-depth study of cellular heterogeneity of rare cells (e.g. circulating tumour cells (CTCs) and circulating foetal cells (CFCs)) is greatly needed in disease management but has never been completely explored due to the current technological limitations. We have developed a retrieval method for single-cell detection using a static droplet array (SDA) device through liquid segmentation with almost no sample loss. We explored the potential of using SDA for low sample input and retrieving the cells of interest using everyday laboratory equipment for downstream molecular analysis. This single-cell isolation and retrieval method is low-cost, rapid and provides a solution to the remaining challenge for single rare cell detection. The entire process takes less than 15 min, is easy to fabricate and allows for on-chip analysis of cells in nanolitre droplets and retrieval of desired droplets. To validate the applicability of our device and method, we mimicked detection of single CTCs by isolating and retrieving single cells and perform real-time PCR on their mRNA contents. Graphical abstract: [Figure not available: see fulltext.]

Cancer Cellular heterogeneity Circulating tumour cells Droplet microfluidics Microfluidic system Single cell detection

SCI

英语

通讯

WOS:000669867200001

CHEMISTRY

2-s2.0-85109282801

Scopus

1.School of Biomedical Engineering,University of Technology Sydney,Sydney,2007,Australia
2.Institute for Biomedical Materials and Devices (IBMD),Faculty of Science,University of Technology Sydney,Sydney,2007,Australia
3.SUStech-UTS Joint Research Centre for Biomedical Materials and Devices,Southern University of Science and Technology,Shenzhen,518055,China
4.Institute of Molecular Medicine,Sechenov First Moscow State University,Moscow,119991,Russian Federation

Ding，Lin,Radfar，Payar,Rezaei，Meysam,et al. An easy-to-operate method for single-cell isolation and retrieval using a microfluidic static droplet array[J]. MICROCHIMICA ACTA,2021,188(8).

Ding，Lin,Radfar，Payar,Rezaei，Meysam,&Warkiani，Majid Ebrahimi.(2021).An easy-to-operate method for single-cell isolation and retrieval using a microfluidic static droplet array.MICROCHIMICA ACTA,188(8).

Ding，Lin,et al."An easy-to-operate method for single-cell isolation and retrieval using a microfluidic static droplet array".MICROCHIMICA ACTA 188.8(2021).