Altered oral and gut microbiota and its association with SARS-CoV-2 viral load in COVID-19 patients during hospitalization

Wu, Yongjian1,2,3,4,5,6,7; Cheng, Xiaomin2; Jiang, Guangmin1,3,4; Tang, Huishu1,3,4; Ming, Siqi1,3,4,6,7; Tang, Lantian1,3,4; Lu, Jiahai2; Guo, Cheng8; Shan, Hong3,4,5; Huang, Xi1,3,4,5,6,7

2021-07-22

10.1038/s41522-021-00232-5

NPJ BIOFILMS AND MICROBIOMES   影响因子和分区

2055-5008

The human oral and gut commensal microbes play vital roles in the development and maintenance of immune homeostasis, while its association with susceptibility and severity of SARS-CoV-2 infection is barely understood. In this study, we investigated the dynamics of the oral and intestinal flora before and after the clearance of SARS-CoV-2 in 53 COVID-19 patients, and then examined their microbiome alterations in comparison to 76 healthy individuals. A total of 140 throat swab samples and 81 fecal samples from these COVID-19 patients during hospitalization, and 44 throat swab samples and 32 fecal samples from sex and age-matched healthy individuals were collected and then subjected to 16S rRNA sequencing and viral load inspection. We found that SARS-CoV-2 infection was associated with alterations of the microbiome community in patients as indicated by both alpha and beta diversity indexes. Several bacterial taxa were identified related to SARS-CoV-2 infection, wherein elevated Granulicatella and Rothia mucilaginosa were found in both oral and gut microbiome. The SARS-CoV-2 viral load in those samples was also calculated to identify potential dynamics between COVID-19 and the microbiome. These findings provide a meaningful baseline for microbes in the digestive tract of COVID-19 patients and will shed light on new dimensions for disease pathophysiology, potential microbial biomarkers, and treatment strategies for COVID-19.

SCI

英语

ESI高被引

通讯

National Natural Science Foundation of China[82072062] ; National Science and Technology Key Projects for Major Infectious Diseases[2017ZX10302301-002] ; Guangzhou Science and Technology Planning Project[201704020226,201604020006] ; National Key Research and Development Program of China[2016YFC1200105] ; Three Major Scientific Research Projects of Sun Yat-sen University[20200326236] ; Guangdong Scientific and Technological Research Project for COVID-19 containment["2020A111128022","2020B111112003"] ; Guangdong Scientific and Technological Research for COVID-19[202020012612200001] ; Zhuhai Scientific and Technological Research Project for COVID-19 containment[ZH22036302200029PWC]

Biotechnology & Applied Microbiology ; Microbiology

Biotechnology & Applied Microbiology ; Microbiology

WOS:000678972600001

NATURE RESEARCH

Web of Science

1.Sun Yat Sen Univ, Affiliated Hosp 1, Ctr Infect & Immun, Zhuhai, Guangdong, Peoples R China
2.Sun Yat Sen Univ, Sch Publ Hlth, Guangzhou, Guangdong, Peoples R China
3.Sun Yat Sen Univ, Affiliated Hosp 1, Guangdong Prov Engn Res Ctr Mol Imaging, Guangdong Prov Key Lab Biomed Imaging, Zhuhai, Guangdong, Peoples R China
4.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Intervent Med, Zhuhai, Guangdong, Peoples R China
5.Southern Marine Sci & Engn Guangdong Lab, Zhuhai, Guangdong, Peoples R China
6.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Shenzhen, Guangdong, Peoples R China
7.Southern Univ Sci & Technol, Affiliated Hosp 2, Shenzhen, Guangdong, Peoples R China
8.Columbia Univ, Mailman Sch Publ Hlth, Ctr Infect & Immun, New York, NY 10027 USA

Wu, Yongjian,Cheng, Xiaomin,Jiang, Guangmin,et al. Altered oral and gut microbiota and its association with SARS-CoV-2 viral load in COVID-19 patients during hospitalization[J]. NPJ BIOFILMS AND MICROBIOMES,2021,7(1).

Wu, Yongjian.,Cheng, Xiaomin.,Jiang, Guangmin.,Tang, Huishu.,Ming, Siqi.,...&Huang, Xi.(2021).Altered oral and gut microbiota and its association with SARS-CoV-2 viral load in COVID-19 patients during hospitalization.NPJ BIOFILMS AND MICROBIOMES,7(1).

Wu, Yongjian,et al."Altered oral and gut microbiota and its association with SARS-CoV-2 viral load in COVID-19 patients during hospitalization".NPJ BIOFILMS AND MICROBIOMES 7.1(2021).