南方科技大学知识苑(SUSTech KC): CircRtn4 Acts as the Sponge of miR-24-3p to Promote Neurite Growth by Regulating CHD5

题名	CircRtn4 Acts as the Sponge of miR-24-3p to Promote Neurite Growth by Regulating CHD5
作者	Qi，Yue 1; Ma，Nana 1; Chen，Xiaofan 1; Wang，Yue 1; Zhang，Wei 1,2; Wan，Jun1,2,3
通讯作者	Zhang，Wei
发表日期	2021-07-07
DOI	10.3389/fnmol.2021.660429
发表期刊	Frontiers in Molecular Neuroscience 影响因子和分区
ISSN	1662-5099
卷号	14
摘要	Circular RNAs (circRNAs) are covalently closed single-stranded RNA molecules. After derived from precursor mRNA back-splicing, circRNAs play important roles in many biological processes. Recently, it was shown that several circRNAs were enriched in the mammalian brain with unclear functions. The expression of circRtn4 in the mouse brain was increased with the differentiation of primary neurons. In our study, knockdown of circRtn4 inhibited neurite growth, while overexpression of circRtn4 significantly increased neurite length. By dual-luciferase reporter assay and RNA antisense purification assay, circRtn4 was identified as a miRNA sponge for miR-24-3p. Moreover, knockdown of miR-24-3p increased neurite length, while overexpression of miR-24-3p significantly inhibited neurite growth. Furthermore, CHD5 was confirmed to be a downstream target gene of miR-24-3p. And CHD5 silence counteracted the positive effect of circRtn4 overexpression on neurite growth. In conclusion, circRtn4 may act as the sponge for miR-24-3p to promote neurite growth by regulating CHD5.
关键词	CHD5 circRtn4 miR-24-3p neurite growth neurodevelopment primary cortical neuron
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	其他
WOS记录号	WOS:000675004700001
Scopus记录号	2-s2.0-85111030491
来源库	Scopus
引用统计	被引频次[WOS]：5
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/242091
专题	生命科学学院_生物系生命科学学院
作者单位	1.Shenzhen Key Laboratory for Neuronal Structural Biology,Biomedical Research Institute,Shenzhen Peking University,The Hong Kong University of Science and Technology Medical Center,Shenzhen,China 2.Greater Bay Biomedical Innocenter,Shenzhen Bay Laboratory,Shenzhen,China 3.Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,China
推荐引用方式 GB/T 7714	Qi，Yue,Ma，Nana,Chen，Xiaofan,et al. CircRtn4 Acts as the Sponge of miR-24-3p to Promote Neurite Growth by Regulating CHD5[J]. Frontiers in Molecular Neuroscience,2021,14.
APA	Qi，Yue,Ma，Nana,Chen，Xiaofan,Wang，Yue,Zhang，Wei,&Wan，Jun.(2021).CircRtn4 Acts as the Sponge of miR-24-3p to Promote Neurite Growth by Regulating CHD5.Frontiers in Molecular Neuroscience,14.
MLA	Qi，Yue,et al."CircRtn4 Acts as the Sponge of miR-24-3p to Promote Neurite Growth by Regulating CHD5".Frontiers in Molecular Neuroscience 14(2021).