Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins

Jia，Ning1,2; Patel，Dinshaw J.1

2021

10.1038/s41580-021-00371-9

NATURE REVIEWS MOLECULAR CELL BIOLOGY   影响因子和分区

1471-0072

1471-0080

CRISPR loci and Cas proteins provide adaptive immunity in prokaryotes against invading bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum of anti-CRISPR proteins (anti-CRISPRs) to counteract and overcome this immunity pathway. Numerous anti-CRISPRs have been identified to date, which suppress single-subunit Cas effectors (in CRISPR class 2, type II, V and VI systems) and multisubunit Cascade effectors (in CRISPR class 1, type I and III systems). Crystallography and cryo-electron microscopy structural studies of anti-CRISPRs bound to effector complexes, complemented by functional experiments in vitro and in vivo, have identified four major CRISPR–Cas suppression mechanisms: inhibition of CRISPR–Cas complex assembly, blocking of target binding, prevention of target cleavage, and degradation of cyclic oligonucleotide signalling molecules. In this Review, we discuss novel mechanistic insights into anti-CRISPR function that have emerged from X-ray crystallography and cryo-electron microscopy studies, and how these structures in combination with function studies provide valuable tools for the ever-growing CRISPR–Cas biotechnology toolbox, to be used for precise and robust genome editing and other applications.

SCI

英语

通讯

WOS:000658135600001

MOLECULAR BIOLOGY & GENETICS

2-s2.0-85107449971

Scopus

1.Structural Biology Program,Memorial Sloan Kettering Cancer Center,New York,United States
2.Department of Biochemistry,School of Medicine,Southern University of Science and Technology,Shenzhen,China

Jia，Ning,Patel，Dinshaw J.. Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins[J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY,2021.

Jia，Ning,&Patel，Dinshaw J..(2021).Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins.NATURE REVIEWS MOLECULAR CELL BIOLOGY.

Jia，Ning,et al."Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins".NATURE REVIEWS MOLECULAR CELL BIOLOGY (2021).