Genome-wide identification of the Capsicum bHLH transcription factor family: discovery of a candidate regulator involved in the regulation of species-specific bioactive metabolites

Liu，Renjian1; Song，Jiali1; Liu，Shaoqun1,2; Chen，Changming1,2; Zhang，Shuanglin1; Wang，Juntao1; Xiao，Yanhui3; Cao，Bihao1,2; Lei，Jianjun1,2,3; Zhu，Zhangsheng1,2,4

2021-12-01

10.1186/s12870-021-03004-7

BMC Plant Biology   影响因子和分区

1471-2229

Background: The basic helix–loop–helix (bHLH) transcription factors (TFs) serve crucial roles in regulating plant growth and development and typically participate in biological processes by interacting with other TFs. Capsorubin and capsaicinoids are found only in Capsicum, which has high nutritional and economic value. However, whether bHLH family genes regulate capsorubin and capsaicinoid biosynthesis and participate in these processes by interacting with other TFs remains unknown. Results: In this study, a total of 107 CabHLHs were identified from the Capsicum annuum genome. Phylogenetic tree analysis revealed that these CabHLH proteins were classified into 15 groups by comparing the CabHLH proteins with Arabidopsis thaliana bHLH proteins. The analysis showed that the expression profiles of CabHLH009, CabHLH032, CabHLH048, CabHLH095 and CabHLH100 found in clusters C1, C2, and C3 were similar to the profile of carotenoid biosynthesis in pericarp, including zeaxanthin, lutein and capsorubin, whereas the expression profiles of CabHLH007, CabHLH009, CabHLH026, CabHLH063 and CabHLH086 found in clusters L5, L6 and L9 were consistent with the profile of capsaicinoid accumulation in the placenta. Moreover, CabHLH007, CabHLH009, CabHLH026 and CabHLH086 also might be involved in temperature-mediated capsaicinoid biosynthesis. Yeast two-hybrid (Y2H) assays demonstrated that CabHLH007, CabHLH009, CabHLH026, CabHLH063 and CabHLH086 could interact with MYB31, a master regulator of capsaicinoid biosynthesis. Conclusions: The comprehensive and systematic analysis of CabHLH TFs provides useful information that contributes to further investigation of CabHLHs in carotenoid and capsaicinoid biosynthesis.

BHLH Capsaicinoids Carotenoids Peppers Temperature Yeast two-hybrid assays

SCI

英语

其他

WOS:000662710800003

PLANT & ANIMAL SCIENCE

2-s2.0-85107558698

Scopus

1.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China),College of Horticulture,Ministry of Agriculture and Rural Affairs,South China Agricultural University,Guangzhou,510642,China
2.Lingnan Guangdong Laboratory of Modern Agriculture,Guangzhou,510642,China
3.Henry Fok College of Biology and Agriculture,Shaoguan University,Shaoguan,512005,China
4.Department of Biology,Peking University-Southern University of Science and Technology Joint Institute of Plant and Food Sciences,Southern University of Science and Technology,Shenzhen,518055,China

Liu，Renjian,Song，Jiali,Liu，Shaoqun,et al. Genome-wide identification of the Capsicum bHLH transcription factor family: discovery of a candidate regulator involved in the regulation of species-specific bioactive metabolites[J]. BMC Plant Biology,2021,21(1).

Liu，Renjian.,Song，Jiali.,Liu，Shaoqun.,Chen，Changming.,Zhang，Shuanglin.,...&Zhu，Zhangsheng.(2021).Genome-wide identification of the Capsicum bHLH transcription factor family: discovery of a candidate regulator involved in the regulation of species-specific bioactive metabolites.BMC Plant Biology,21(1).

Liu，Renjian,et al."Genome-wide identification of the Capsicum bHLH transcription factor family: discovery of a candidate regulator involved in the regulation of species-specific bioactive metabolites".BMC Plant Biology 21.1(2021).