Point-of-care immunoassays with tunable detection range for detecting infection in intensive care unit

Mou，Lei1,2; Li，Zulan3; Qi，Jie1,2; Jiang，Xingyu1,2

2021-05-01

10.31635/ccschem.020.202000331

CCS Chemistry   影响因子和分区

2096-5745

2096-5745

One of the difficulties of a multiplexed analytical assay is matching the concentration range of different markers. Here, the authors develop an automatic, multiplexed point-of-care (POC) immunoassay that allows detecting C-reactive protein (CRP), procalcitonin (PCT), and interleukin 6 (IL-6) in 50 μL serum samples with limits of detection 1.87 μg/mL, 0.17 ng/mL, and 49.75 pg/mL, respectively. The authors use electrospun fibers and surface chemistry of gold nanoparticles (AuNPs) to adjust the detection range of different biomarkers. The authors have proposed some new diagnostic indicators (mScore and mScorePlus) that combine the results of CRP, PCT, IL-6, and complete blood counts (CBCs). The authors also tracked changes in CRP, PCT, and IL-6 of patients in an intensive care unit (ICU). The authors find that mScore and mScorePlus have advantages in improving the diagnostic accuracy and providing more analytical information. mScore and mScorePlus are effective tools to detect infections, differentiate bacterial and viral infections, and monitor disease. Integrating multiple markers into one straightforward parameter is an effective method for analytical applications. The authors believe that this method provides a general way for chemists to develop increasingly accurate detection methods and indicators.

Immunoassay Intensive care unit Microfluidics Multiplexed assay Point-of-care testing

ESCI

英语

第一 ; 通讯

National Key R&D Program of China["2018YFA0902600","2017YFA0205901"] ; National Natural Science Foundation of China[21535001,81730051,21761142006] ; Chinese Academy of Sciences["QYZDJ-SSW-SLH039","121D11KYSB20170026","XDA16020902"] ; Shenzhen Bay Laboratory[SZBL2019062801004] ; Guangdong Innovative and Entrepreneurial Research Team Program[2019ZT08Y191]

Chemistry

Chemistry, Multidisciplinary

WOS:000794145300016

CHINESE CHEMICAL SOC

2-s2.0-85107935019

Scopus

1.Department of Biomedical Engineering,Southern University of Science and Technology,Guangdong,518055,China
2.National Center for NanoScience and Technology,University of Chinese Academy of Sciences,Beijing,100190,China
3.Reproduction Center of the 306th Hospital of People's Liberation Army,Beijing,100101,China

Mou，Lei,Li，Zulan,Qi，Jie,et al. Point-of-care immunoassays with tunable detection range for detecting infection in intensive care unit[J]. CCS Chemistry,2021,3(5):1562-1572.

Mou，Lei,Li，Zulan,Qi，Jie,&Jiang，Xingyu.(2021).Point-of-care immunoassays with tunable detection range for detecting infection in intensive care unit.CCS Chemistry,3(5),1562-1572.

Mou，Lei,et al."Point-of-care immunoassays with tunable detection range for detecting infection in intensive care unit".CCS Chemistry 3.5(2021):1562-1572.