Comparative Proteomic Analysis to Investigate the Pathogenesis of Oral Adenoid Cystic Carcinoma

Li, Wen1,2,3,4; Zhang, Qian1,2,3,4; Wang, Xiaobin1,2,3,4; Wang, Hanlin4; Zuo, Wenxin5; Xie, Hongliang5; Tang, Jianming5; Wang, Mengmeng5; Zeng, Zhipeng5; Cai, Wanxia5; Tang, Donge5; Dai, Yong5

2021-07-27

10.1021/acsomega.1c01270

ACS Omega   影响因子和分区

2470-1343

Adenoid cystic carcinoma ( ACC) belongs to salivary gland malignancies commonly occurring in an oral cavity with a poor long-term prognosis. The potential biomarkers and cellular functions acting on local recurrences and distant metastases remain to be illustrated. Proteomics is the core content of precision medicine research, which provides accurate information for early detection of cancer, benign and malignant diagnosis, classification and personalized medication, efficacy monitoring, and prognosis judgment. To obtain a comprehensive regulation network and supply clues for the treatment of oral ACC (OACC), we utilized mass spectrometry-based quantitative proteomics to analyze the protein expression profile in paired tumor and adjacent normal tissues. We identified a total of 40,547 specific peptides and 4454 differentially expressed proteins (DEPs), in which HAPLN1 was the most upregulated protein and BPIFB1 was the most downregulated. Then, we annotated the functions and characteristics of DEPs in detail from the aspects of gene ontology, subcellular structural localization, KEGG, and protein domain to thoroughly understand the identified and quantified proteins. Glycosphingolipid biosynthesis and glycosaminoglycan degradation pathways showed the biggest difference according to KEGG analysis. Moreover, we confirmed 20 proteins from the ECM-receptor signaling pathway by a parallel reaction monitoring quantitative detection and 19 proteins were quantified. This study provides useful insights to analyze DEPs in OACC and guide indepth thinking of the pathogenesis from a proteomics view for anticancer mechanisms and potential biomarkers.

SCI

英语

通讯

Science, Technology, and Innovation Commission of Shenzhen Municipality["JCYJ20190807145815129","JCYJ20180306140810282"] ; China Postdoctoral Science Foundation["2020M670047ZX","2020M670048ZX","2020M682888"]

Chemistry

Chemistry, Multidisciplinary

WOS:000679374200008

AMER CHEMICAL SOC

Web of Science

1.Shenzhen Univ, Gen Hosp, Carson Int Canc Ctr, Shenzhen 518000, Guangdong, Peoples R China
2.Shenzhen Univ, Clin Med Acad Ctr, Shenzhen 518000, Guangdong, Peoples R China
3.Shenzhen Univ, Coll Phys & Optoelect Engn, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Peoples R China
4.Shenzhen Univ, Hlth Sci Ctr, Sch Med, Shenzhen 518060, Peoples R China
5.Southern Univ Sci & Technol, Clin Med Res Ctr,Affiliated Hosp 1,Jinan Univ, Shenzhen Peoples Hosp,Guangdong Prov Engn Res Ctr, Shenzhen Engn Res Ctr Autoimmune Dis,Clin Med Col, Shenzhen 518020, Guangdong, Peoples R China

Li, Wen,Zhang, Qian,Wang, Xiaobin,et al. Comparative Proteomic Analysis to Investigate the Pathogenesis of Oral Adenoid Cystic Carcinoma[J]. ACS Omega,2021,6(29).

Li, Wen.,Zhang, Qian.,Wang, Xiaobin.,Wang, Hanlin.,Zuo, Wenxin.,...&Dai, Yong.(2021).Comparative Proteomic Analysis to Investigate the Pathogenesis of Oral Adenoid Cystic Carcinoma.ACS Omega,6(29).

Li, Wen,et al."Comparative Proteomic Analysis to Investigate the Pathogenesis of Oral Adenoid Cystic Carcinoma".ACS Omega 6.29(2021).