Dysregulated hematopoiesis in bone marrow marks severe COVID-19

Wang, Xin1,2,3; Wen, Yanling1,2,3; Xie, Xiaowei4,5,6,7; Liu, Yang1,2; Tan, Xiaohua8; Cai, Qingxian9; Zhang, Yawen4,5,6,7; Cheng, Lin1,2; Xu, Gang1,2; Zhang, Shengyuan1,2,3; Wang, Haiyan1,2; Wei, Lanlan1,2; Tang, Xian1,2; Qi, Furong1,2,3; Zhao, Juanjuan1,2,10; Yuan, Jing11; Liu, Lei1,2; Zhu, Ping4,5,6,7; Ginhoux, Florent12; Zhang, Shuye13; Cheng, Tao4,5,6,7; Zhang, Zheng1,2,10,14

2021-08-04

10.1038/s41421-021-00296-9

CELL DISCOVERY   影响因子和分区

2056-5968

Severe coronavirus disease 2019 (COVID-19) is often indicated by lymphopenia and increased myelopoiesis; however, the underlying mechanism is still unclear, especially the alteration of hematopoiesis. It is important to explore to what extent and how hematopoietic stem cells contribute to the impairment of peripheral lymphoid and myeloid compartments in COVID-19 patients. In this study, we used single-cell RNA sequencing to assess bone marrow mononuclear cells from COVID-19 patients with peripheral blood mononuclear cells as control. The results showed that the hematopoietic stem cells in these patients were mainly in the G1 phase and prone to apoptosis, with immune activation and anti-viral responses. Importantly, a significant accumulation of immature myeloid progenitors and a dramatic reduction of lymphoid progenitors in severe cases were identified, along with the up-regulation of transcription factors (such as SPI1, LMO4, ETS2, FLI1, and GATA2) that are important for the hematopoietic stem cell or multipotent progenitor to differentiate into downstream progenitors. Our results indicate a dysregulated hematopoiesis in patients with severe COVID-19.

SCI

英语

第一 ; 通讯

National Science Fund for Distinguished Young Scholars[82025022] ; Shenzhen Science and Technology Innovation Committee["2020A1111350032","JSGG2020022515141 0198","JCYJ20200109144201725"] ; Central Charity Fund of Chinese Academy of Medical Science[2020-PT310-009] ; Shenzhen Bay Funding[2020B1111340075] ; Guangdong Provincial Department of Science and Technology["2020B1111340033","2020B1111340039","2020B1111340040"] ; Ministry of Science and Technology of China[2016YFA0100600] ; National Natural Science Foundation of China[81421002,8210081333] ; China Postdoctoral Science Foundation[2021M693357] ; CAMS Initiative for Innovative Medicine[2016-I2M-1017]

Cell Biology

Cell Biology

WOS:000681323100001

SPRINGERNATURE

Web of Science

1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Inst Hepatol, Shenzhen, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 2, Sch Med, Shenzhen, Guangdong, Peoples R China
3.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing, Peoples R China
4.Chinese Acad Med Sci & Peking Union Med Coll, Inst Hematol & Blood Dis Hosp, State Key Lab Expt Hematol, Tianjin, Peoples R China
5.Chinese Acad Med Sci & Peking Union Med Coll, Inst Hematol & Blood Dis Hosp, Natl Clin Res Ctr Blood Dis, Tianjin, Peoples R China
6.Chinese Acad Med Sci & Peking Union Med Coll, Ctr Stem Cell Med, Tianjin, Peoples R China
7.Chinese Acad Med Sci & Peking Union Med Coll, Dept Stem Cell & Regenerat Med, Tianjin, Peoples R China
8.Shenzhen Third Peoples Hosp, Dept Oncol & Hematol, Shenzhen, Guangdong, Peoples R China
9.Shenzhen Third Peoples Hosp, Dept Hepatol, Shenzhen, Guangdong, Peoples R China
10.Shenzhen Bay Lab, Shenzhen, Guangdong, Peoples R China
11.Shenzhen Third Peoples Hosp, Dept Infect Dis, Shenzhen, Guangdong, Peoples R China
12.ASTAR, Singapore Immunol Network SIgN, Biopolis, Singapore, Singapore
13.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China
14.Chinese Acad Med Sci, Shenzhen Res Ctr Communicable Dis Diag & Treatmen, Shenzhen, Guangdong, Peoples R China

Wang, Xin,Wen, Yanling,Xie, Xiaowei,et al. Dysregulated hematopoiesis in bone marrow marks severe COVID-19[J]. CELL DISCOVERY,2021,7(1).

Wang, Xin.,Wen, Yanling.,Xie, Xiaowei.,Liu, Yang.,Tan, Xiaohua.,...&Zhang, Zheng.(2021).Dysregulated hematopoiesis in bone marrow marks severe COVID-19.CELL DISCOVERY,7(1).

Wang, Xin,et al."Dysregulated hematopoiesis in bone marrow marks severe COVID-19".CELL DISCOVERY 7.1(2021).