Digital hybridization human papillomavirus assay with attomolar sensitivity without amplification

Mou，Lei1,2; Hong，Honghai1; Xu，Xiaojian2; Xia，Yong1; Jiang，Xingyu1,2

2021

10.1021/acsnano.1c02311

ACS Nano   影响因子和分区

1936-0851

1936-086X

Detection of nucleic acid without amplification can avoid problems associated with thermal cycling such as labor-intensiveness and aerosol pollution. Here we develop a droplet-based digital microfluidic hybridization assay for nucleic acid detection with attomolar sensitivity. This assay provides a clinically useful sensitivity for detecting human papillomavirus (HPV) without amplification. The sensitivity is accomplished using femtoliter-sized droplet microfluidics for concentrating enzyme-catalyzed fluorescent products into a detectable signal and magnetic beads for accelerating reaction time. Meanwhile, using magnetic beads and droplet microfluidic chips, we can improve the sampling efficiency over conventional methods. We characterized the sensitivity, selectivity, detection range, stability, and accuracy of our assay. Our assay is 50-fold more sensitive than the traditional hybrid capture assay. The assay without amplification avoids problems of complex handling procedures and aerosol pollution. The direct and sensitive detection of nucleic acid using a droplet microfluidic system provides an early disease diagnosis tool.

Amplification-free Bioassay Digital hybridization assay Droplet microfluidics Human papillomavirus Hybrid capture

SCI ; EI

英语

NI论文

其他

National Key R&D Program of China["2018YFA0902600","2017YFA0205901"] ; National Natural Science Foundation of China[21535001,81730051,21761142006] ; Chinese Academy of Sciences["QYZDJSSW-SLH039","121D11KYSB20170026","XDA16020902"] ; Shenzhen Bay Laboratory[SZBL2019062801004] ; Guangdong Innovative and Entrepreneurial Research Team Program[2019ZT08Y191] ; Guangzhou Municipal Science and Technology Bureau[202002020085]

Chemistry ; Science & Technology - Other Topics ; Materials Science

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000693105500043

AMER CHEMICAL SOC

20213310780960

Aerosols ; Air pollution ; Biomolecules ; Diagnosis ; Digital microfluidics ; Drops ; Fluidic devices ; Human reaction time ; Nucleic acids ; Thermal pollution

Air Pollution:451 ; Bioengineering and Biology:461 ; Hydraulic Equipment and Machinery:632.2 ; Microfluidics:632.5.1

2-s2.0-85112531749

Scopus

1.Department of Clinical Laboratory,Third Affiliated Hospital of Guangzhou Medical University,Guangzhou,No. 63 Duobao Road, Liwan District, Guangdong,510150,China
2.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,No. 1088, Xueyuan Road, Xili, Nanshan District, Guangdong,518055,China

Mou，Lei,Hong，Honghai,Xu，Xiaojian,et al. Digital hybridization human papillomavirus assay with attomolar sensitivity without amplification[J]. ACS Nano,2021,15(8):13077-13084.

Mou，Lei,Hong，Honghai,Xu，Xiaojian,Xia，Yong,&Jiang，Xingyu.(2021).Digital hybridization human papillomavirus assay with attomolar sensitivity without amplification.ACS Nano,15(8),13077-13084.

Mou，Lei,et al."Digital hybridization human papillomavirus assay with attomolar sensitivity without amplification".ACS Nano 15.8(2021):13077-13084.