Signal Transducer and Activator of Transcription6 Signaling Pathway in Tumor-Associated Macrophage Aggravates Lung Cancer Progression

Wan, Jun; Wang, Jian; Huang, Qiurong; Ding, Guanggui; Ling, Xiean

2021-09-01

10.1166/jbt.2021.2599

Journal of Biomaterials and Tissue Engineering   影响因子和分区

2157-9083

2157-9091

Lung cancer is a most common cancer worldwide. Tumor-associated macrophage (TAM) is known a key effector cell in tumor microenvironment. Meanwhile, STAT6 is crucial to cancer development. We aimed to determine the interaction between STAT6 and TAMs in lung cancer. In this work, firstly, we established mouse model of lung cancer. Then, immunofluorescence was performed to determine STAT6 and CD206 level in lung cancer tissue and adjacent normal tissues as well as model mice. RT-qPCR was applied to detect differentiation of macrophage and determine related gene expression. After treatment of siRNA of STAT6 or STAT6 inhibitor (AS1517499), Transwell assay and MTT were used to determine cell proliferation and migration. STAT6 was upregulated in lung cancer tissues while arginase was more active in M2 macrophage rather than M1 macrophage. Transfection of si-STAT6 not only decreased differentiation in M2 macrophage but also inhibited proliferative, migratory and invasive ability of cancer cells while AS1517499 led to reduced tumor growth. STAT6 inhibition caused decreased expression of M2 macrophages. Similarly, intratumoral T cell markers showed that CD8+ T cell gene expression and CD4-mediated T cell marker FoxP3 was increased slightly. Taken altogether, macrophage-STAT6 promotes cell migration and proliferation in lung cancer.

STAT6 Lung Cancer Macrophage M2 CD8(+)

SCI

英语

第一 ; 通讯

Natural Science Foundation of Guangdong Province[2018A0303130247]

Cell Biology

Cell & Tissue Engineering

WOS:000682852000007

AMER SCIENTIFIC PUBLISHERS

Web of Science

Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2,Affiliated Hosp 1,Dept Thorac Sur, Shenzhen 518020, Guangdong, Peoples R China

Wan, Jun,Wang, Jian,Huang, Qiurong,et al. Signal Transducer and Activator of Transcription6 Signaling Pathway in Tumor-Associated Macrophage Aggravates Lung Cancer Progression[J]. Journal of Biomaterials and Tissue Engineering,2021,11(9).

Wan, Jun,Wang, Jian,Huang, Qiurong,Ding, Guanggui,&Ling, Xiean.(2021).Signal Transducer and Activator of Transcription6 Signaling Pathway in Tumor-Associated Macrophage Aggravates Lung Cancer Progression.Journal of Biomaterials and Tissue Engineering,11(9).

Wan, Jun,et al."Signal Transducer and Activator of Transcription6 Signaling Pathway in Tumor-Associated Macrophage Aggravates Lung Cancer Progression".Journal of Biomaterials and Tissue Engineering 11.9(2021).