Effects of pore size and porosity on cytocompatibility and osteogenic differentiation of porous titanium

Yao, Yi-Tong1; Yang, Yue2,3; Ye, Qi4; Cao, Shan-Shan5; Zhang, Xin-Ping5; Zhao, Ke1; Jian, Yutao6

2021-06-01

10.1007/s10856-021-06548-0

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE   影响因子和分区

0957-4530

1573-4838

To find out the optimal porosity and pore size of porous titanium (Ti) regarding the cytocompatibility and osteogenic differentiation. Six groups of porous Ti samples with different porosities and pore sizes were fabricated by the powder metallurgy process. The microstructure and compressive mechanical properties were characterized. The cytocompatibility was examined by a series of biological tests as protein absorption with BCA assay kit, cell attachment with laser scanning confocal microscopy and vinculin expression, cell proliferation with CCK-8 assay. Cell differentiation and calcification were detected by qPCR and Alizarin Red S dying respectively. Pores distributed homogeneously throughout the porous Ti samples. The compressive test results showed that Young's modulus ranged from 2.80 +/- 0.03 GPa to 5.43 +/- 0.34 GPa and the compressive strength increased from 112.4 +/- 3.6 MPa to 231.1 +/- 9.4 MPa. Porous Ti with high porosity (53.3 +/- 1.2%) and small pore size (191.6 +/- 3.7 mu m) adsorbed more proteins. More MC3T3-E1 cells adhered onto dense Ti samples than onto any other porous ones already after culture and no difference was identified within the porous groups. The porous structure of porous Ti with a porosity of 53.3 +/- 1.2% and an average pore size of 191.6 +/- 3.7 mu m facilitated cell differentiation and calcification. Small pores were not beneficial to the osteo-initiation at the very beginning. Porous Ti with a porosity of 53.3 +/- 1.2% and an average pore size of 191.6 +/- 3.7 mu m fabricated by powder metallurgy process showed the expected mechanical property and improved osseointegration as implants in dental treatment.

SCI ; EI

英语

其他

National Natural Science Foundation of China[81771110,82071156] ; Guangdong Provincial Natural Science Foundation Key Project["2018B0303110012","2017A030313323"]

Engineering ; Materials Science

Engineering, Biomedical ; Materials Science, Biomaterials

WOS:000691278100002

SPRINGER

MATERIALS SCIENCE

Web of Science

1.Sun Yat Sen Univ, Hosp Stomatol, Guanghua Sch Stomatol, Guangdong Prov Key Lab Stomatol, Guangzhou, Peoples R China
2.Jinan Univ, Dept Stomatol, Shenzhen Peoples Hosp, Clin Med Sch 2, Shenzhen, Peoples R China
3.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen, Peoples R China
4.Jinan Univ, Shenzhen Baoan Womens & Childrens Hosp, Shenzhen, Peoples R China
5.South China Univ Technol, Sch Mat Sci & Engn, Guangzhou, Peoples R China
6.Sun Yat Sen Univ, Inst Stomatol Res, Guangdong Prov Key Lab Stomatol, Guangzhou, Peoples R China

Yao, Yi-Tong,Yang, Yue,Ye, Qi,et al. Effects of pore size and porosity on cytocompatibility and osteogenic differentiation of porous titanium[J]. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE,2021,32(6).

Yao, Yi-Tong.,Yang, Yue.,Ye, Qi.,Cao, Shan-Shan.,Zhang, Xin-Ping.,...&Jian, Yutao.(2021).Effects of pore size and porosity on cytocompatibility and osteogenic differentiation of porous titanium.JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE,32(6).

Yao, Yi-Tong,et al."Effects of pore size and porosity on cytocompatibility and osteogenic differentiation of porous titanium".JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE 32.6(2021).