Characterization of the Genitourinary Microbiome of 1,165 Middle-Aged and Elderly Healthy Individuals

Qin，Junjie1,2,3; Shi，Xulian1; Xu，Junming1,2,3; Yuan，Simin1; Zheng，Bo2; Zhang，Enpu1; Huang，Guixiao1; Li，Guo1; Jiang，Ganggang1; Gao，Shan1; Tian，Cheng3; Guo，Ruochun3; Fu，Zhicong3; Huang，Qingru3; Yang，Rentao3; Zhang，Wenyong4; Li，Shenghui3; Wu，Song1,5,6

2021-08-19

10.3389/fmicb.2021.673969

Frontiers in Microbiology   影响因子和分区

1664-302X

Accumulated evidence shows that complex microbial communities resides in the healthy human urinary tract and can change in urological disorders. However, there lacks a comprehensive profiling of the genitourinary microbiota in healthy cohort. Here, we performed 16S rRNA gene sequencing of midstream urine specimens from 1,172 middle-aged and elderly healthy individuals. The core microbiota included 6 dominant genera (mean relative abundance >5%), including Prevotella, Streptococcus, Lactobacillus, Gardnerella, Escherichia-Shigella, and Veillonella, and 131 low-abundance genera (0.01–5%), displaying a distinct microbiome profiles to that of host-matched gut microbiota. The composition and diversity of genitourinary microbiome (GM) were distinct between genders and may fluctuate with ages. Several urotypes were identified by the stratification of microbiome profiles, which were mainly dominated by the six most predominant genera. The prevalence of urotypes was disparate between genders, and the male sample additionally harbored other urotypes dominated by Acinetobacter, Corynebacterium, Staphylococcus, or Sphingomonas. Peptoniphilus, Ezakiella, and Porphyromonas were co-occurred and co-abundant, and they may play crucial roles as keystone genera and be associated with increased microbial diversity. Our results delineated the microbial structure and diversity landscape of the GM in healthy middle-aged and elderly adults and provided insights into the influence of gender and age to it.

co-occurrence network core microbiota genitourinary microbiome microbial diversity urotypes

SCI

英语

其他

WOS:000692979800001

2-s2.0-85114300355

Scopus

1.Department of Urology,Hospital of Shenzhen University (Luohu Hospital Group),Shenzhen,China
2.State Key Laboratory of Chemical Oncogenomics,Key Laboratory of Chemical Biology,Tsinghua Shenzhen International Graduate School,Shenzhen,China
3.Department of Human Microbiome,Promegene Institute,Shenzhen,China
4.School of Medicine,Southern University of Science and Technology,Shenzhen,China
5.Teaching Center of Shenzhen Luohu Hospital,Shantou University Medical College,Shantou,China
6.Department of Urology and Guangdong Key Laboratory of Urology,Hospital of Guangzhou Medical University,Guangzhou,China

Qin，Junjie,Shi，Xulian,Xu，Junming,et al. Characterization of the Genitourinary Microbiome of 1,165 Middle-Aged and Elderly Healthy Individuals[J]. Frontiers in Microbiology,2021,12.

Qin，Junjie.,Shi，Xulian.,Xu，Junming.,Yuan，Simin.,Zheng，Bo.,...&Wu，Song.(2021).Characterization of the Genitourinary Microbiome of 1,165 Middle-Aged and Elderly Healthy Individuals.Frontiers in Microbiology,12.

Qin，Junjie,et al."Characterization of the Genitourinary Microbiome of 1,165 Middle-Aged and Elderly Healthy Individuals".Frontiers in Microbiology 12(2021).